嗜热解糖梭菌培养物的生长和代谢动力学。其质粒的分离与特性
Kinetics of growth and metabolism of Clostridium thermosaccharolyticum culture. Isolation and characteristics of its plasmids.
作者信息
Belogurova N G, Mosolova T P, Kalyuzhnyy S V, Varfolomeyev S D
机构信息
Chemistry Department, Moscow State University, USSR.
出版信息
Appl Biochem Biotechnol. 1991 Jan;27(1):1-8. doi: 10.1007/BF02921510.
The kinetics of growth and metabolism of Clostridium thermosaccharolyticum DSM 571 has been studied at varying initial pH and glucose concentration. A weak inhibitory effect of excess glucose on the specific growth rate has been shown. The effect of antibiotics of various classes on culture growth and hydrogen evolution has been studied. Streptomycin and kanamycin resistance of this culture has been revealed as well as the phenomenon of increased hydrogen production in the presence of the above antibiotics. New plasmids, pNB1 (4.9 kb) and pNB2 (2.0 kb), were isolated from C. thermosaccharolyticum DSM 571. The restriction analysis of pNB1 and pNB2 has been performed.
已在不同初始pH值和葡萄糖浓度下研究了嗜热解糖梭菌DSM 571的生长和代谢动力学。研究表明，过量葡萄糖对比生长速率有微弱的抑制作用。研究了各类抗生素对培养物生长和氢气释放的影响。揭示了该培养物对链霉素和卡那霉素的抗性，以及在上述抗生素存在下产氢增加的现象。从嗜热解糖梭菌DSM 571中分离出了新的质粒pNB1（4.9 kb）和pNB2（2.0 kb）。已对pNB1和pNB2进行了限制性分析。
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