Belogurova N G, Del'ver E P, Kaliuzhnyĭ S V, Varfolomeev S D, Rodzevich O V, Belogurov A A
Mol Biol (Mosk). 1992 Jan-Feb;26(1):173-8.
The complete nucleotide sequence of pNB2, a 1.9-kilobases cryptic plasmid from thermophilic Clostridium thermosaccharolyticum has been determined. The plasmid consists of 1882 base pairs and has a G+C composition of 27.2%. The sequence contains three open reading frames capable of coding for polypeptides two of which were identified in maxicell Escherichia coli extracts. Our future studies are directed toward a construction of pNB2-derivatives as vectors for Clostridia.