Morrison A I, Panayotova-Heiermann M, Feigl G, Schölermann B, Kinne R K
Max-Planck-Institut für Systemphysiologie, Dortmund, F.R.G.
Biochim Biophys Acta. 1991 May 2;1089(1):121-3. doi: 10.1016/0167-4781(91)90093-2.
Using an isolated 1.2 kbp PCR product as specific probe, the rabbit renal cortex sodium-D-glucose cotransport system has been found and cloned from a renal cortex cDNA library. Two overlapping cDNA clones were found, one with a length of 2.1 kbp and one of 1.1 kbp resulting in a total sequence of 2.2 kbp without the poly(A) tail. Northern blot hybridization indicated a transcript of approx. 2.4 kb in size. Tissue distribution in the rabbit kidney and intestine indicates that this sequence is present in renal cortex, renal medulla and small intestine in different proportions. It is proposed that this clone codes for a member of the sodium-D-glucose cotransporter systems.
以一个分离的1.2千碱基对的聚合酶链反应（PCR）产物作为特异性探针，已从兔肾皮质cDNA文库中发现并克隆出兔肾皮质钠- D -葡萄糖协同转运系统。发现了两个重叠的cDNA克隆，一个长度为2.1千碱基对，另一个为1.1千碱基对，从而得到一个不含多聚腺苷酸（poly(A)）尾的2.2千碱基对的完整序列。Northern印迹杂交显示转录本大小约为2.4千碱基。兔肾和肠道中的组织分布表明，该序列以不同比例存在于肾皮质、肾髓质和小肠中。推测该克隆编码钠- D -葡萄糖协同转运蛋白系统的一个成员。
