Davidson S F, Brantley S K, Das S K
Department of Surgery, University of Mississippi Medical Center, Jackson.
Br J Plast Surg. 1991 Apr;44(3):210-4. doi: 10.1016/0007-1226(91)90129-8.
The effects of prior ultraviolet light exposure on wound tensile strength and skin histology were evaluated in the hairless guinea pig model. Hairless guinea pigs (strain IAF/HA-HO) were irradiated with either UVA (80 J/cm2) or UVB (0.46 J/cm2) every other day for 16 weeks. Following cessation of treatment, a standard dorsal wound was made in each animal, allowed to heal, and mechanically tested to failure at 21 days. Serial 4 mm punch biopsies were obtained prior to the initial exposure and at 2, 4, 8, 12 and 16 weeks. Histological examination with haematoxylin and eosin, trichrome and elastin stains was performed. In comparison to the unexposed control group, wound tensile strength was significantly less in the UVA- and UVB-irradiated animals. Histological examination revealed a marked endothelial swelling and eosinophilic infiltration in the irradiated groups. These results indicate that repeated exposure to even moderate doses of non-ionising radiation alters normal skin structure and adversely affects subsequent wound tensile strength in this model.
在无毛豚鼠模型中评估了先前紫外线照射对伤口抗张强度和皮肤组织学的影响。无毛豚鼠（IAF/HA-HO品系）每隔一天接受UVA（80 J/cm2）或UVB（0.46 J/cm2）照射，持续16周。治疗停止后，在每只动物身上制作标准背部伤口，使其愈合，并在21天时进行机械测试直至失效。在初次照射前以及第2、4、8、12和16周时获取4毫米系列打孔活检组织。进行苏木精和伊红、三色和弹性蛋白染色的组织学检查。与未照射的对照组相比，UVA和UVB照射动物的伤口抗张强度明显较低。组织学检查显示照射组有明显的内皮肿胀和嗜酸性粒细胞浸润。这些结果表明，在该模型中，即使反复暴露于中等剂量的非电离辐射也会改变正常皮肤结构，并对随后的伤口抗张强度产生不利影响。
