Pryce G, Male D K, Sarkar C
Department of Neuropathology, Institute of Psychiarty, London, U.K.
Immunology. 1991 Mar;72(3):393-8.
We have determined whether particular lymphocyte populations bind preferentially to cerebral endothelium, using adhesion assays and a new method for in situ staining of adherent lymphocytes. B cells bind more strongly than T cells, an effect enhanced by lymphocyte activation or endothelial cell stimulation with interferon-gamma (IFN-gamma) or tumour necrosis factor-alpha (TNF-alpha). This is not equated with levels of CD18 expression on the lymphocytes. CD8+ T cells bound more efficiently than CD4+ cells under all conditions. To determine whether there was a population of cells which selectively homes to the brain, we compared adhesion of cervical lymph nodes cells to brain endothelium, with adhesion of lymphocytes from other nodes. In 50% of the experiments there was significantly enhanced binding of activated cervical lymph cells to cerebral endothelium but not to control (aortic) endothelium. This effect was seen using both normal and IFN-gamma-activated endothelium. The explanation for this finding is that cervical lymph nodes frequently, but not invariably, contain higher proportions of CD8+ cells and B cells than other lymph nodes. These data imply that selective adhesion of lymphocytes to brain endothelium is related to the subpopulations involved and this may be reflected in the cell types seen in immunological lesions of the brain, and in the relative proportions of the subpopulations seen in cervical lymph nodes.
我们使用黏附试验和一种新的原位染色方法来检测贴壁淋巴细胞，以确定特定淋巴细胞群体是否优先与脑内皮细胞结合。B细胞比T细胞结合更紧密，淋巴细胞活化或用γ干扰素（IFN-γ）或肿瘤坏死因子-α（TNF-α）刺激内皮细胞可增强这种效应。这与淋巴细胞上CD18的表达水平无关。在所有条件下，CD8⁺T细胞比CD4⁺细胞结合更有效。为了确定是否存在选择性归巢至脑的细胞群体，我们比较了颈淋巴结细胞与脑内皮细胞的黏附情况以及其他淋巴结淋巴细胞的黏附情况。在50%的实验中，活化的颈淋巴结细胞与脑内皮细胞的结合显著增强，但与对照（主动脉）内皮细胞的结合未增强。使用正常内皮细胞和IFN-γ活化的内皮细胞均观察到这种效应。这一发现的解释是，颈淋巴结通常（但并非总是）比其他淋巴结含有更高比例的CD8⁺细胞和B细胞。这些数据表明，淋巴细胞与脑内皮细胞的选择性黏附与所涉及的亚群有关，这可能反映在脑免疫损伤中所见的细胞类型以及颈淋巴结中所见亚群的相对比例上。
