• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

体外与人微血管内皮细胞结合的淋巴细胞的表型和功能特征。自然杀伤细胞优先结合的证据。

Phenotypic and functional characterization of lymphocytes that bind human microvascular endothelial cells in vitro. Evidence for preferential binding of natural killer cells.

作者信息

Bender J R, Pardi R, Karasek M A, Engleman E G

出版信息

J Clin Invest. 1987 Jun;79(6):1679-88. doi: 10.1172/JCI113007.

DOI:10.1172/JCI113007
PMID:3495552
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC424501/
Abstract

The microvascular endothelium has been postulated to be a critical target in the rejection of vascularized allografts. This study was undertaken to examine the ability of human sheep erythrocyte rosette forming lymphocytes (E-RFC) to form stable conjugates with microvascular endothelial cells (EC), and to assess whether a receptor-ligand interaction mediates this event. Human foreskin microvascular EC monolayers were used as targets of chromium-51-labeled E-RFC in a quantitative adherence assay. Binding was saturable, displaceable by unlabeled E-RFC, augmented by recombinant interleukin 1 (rIL-1) and inhibited by anti-LFA1 antibody. The Leu-11+ lymphocyte subset, known to be enriched for natural killer (NK) cells, bound preferentially. Only the EC-adherent lymphocyte fraction contained NK effectors, which lysed EC and classical NK targets. Thus, NK cells adhere to microvascular EC via a specific receptor-ligand interaction. The possibility exists that such binding occurs in recipients of vascularized allografts, representing the initial stage of graft rejection.

摘要

微血管内皮被认为是血管化同种异体移植物排斥反应中的关键靶点。本研究旨在检测人羊红细胞花环形成淋巴细胞（E-RFC）与微血管内皮细胞（EC）形成稳定结合物的能力，并评估受体-配体相互作用是否介导了这一过程。在定量黏附试验中，用人包皮微血管EC单层作为铬-51标记的E-RFC的靶细胞。结合具有饱和性，可被未标记的E-RFC取代，可被重组白细胞介素1（rIL-1）增强，并被抗LFA1抗体抑制。已知富含自然杀伤（NK）细胞的Leu-11+淋巴细胞亚群优先结合。只有黏附于EC的淋巴细胞部分含有NK效应细胞，这些细胞可裂解EC和经典NK靶细胞。因此，NK细胞通过特异性受体-配体相互作用黏附于微血管EC。在血管化同种异体移植物受者中存在这种结合的可能性，这代表了移植物排斥反应的初始阶段。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4741/424501/8985e5d9cc27/jcinvest00117-0144-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4741/424501/8b4fb65ec029/jcinvest00117-0144-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4741/424501/ad48d03cb6a3/jcinvest00117-0144-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4741/424501/8985e5d9cc27/jcinvest00117-0144-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4741/424501/8b4fb65ec029/jcinvest00117-0144-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4741/424501/ad48d03cb6a3/jcinvest00117-0144-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4741/424501/8985e5d9cc27/jcinvest00117-0144-c.jpg

相似文献

1
Phenotypic and functional characterization of lymphocytes that bind human microvascular endothelial cells in vitro. Evidence for preferential binding of natural killer cells.体外与人微血管内皮细胞结合的淋巴细胞的表型和功能特征。自然杀伤细胞优先结合的证据。
J Clin Invest. 1987 Jun;79(6):1679-88. doi: 10.1172/JCI113007.
2
Lymphocyte subsets differentially induce class II human leukocyte antigens on allogeneic microvascular endothelial cells.淋巴细胞亚群可差异性地诱导同种异体微血管内皮细胞上的II类人类白细胞抗原。
J Immunol. 1987 Oct 15;139(8):2585-92.
3
Recombinant interleukin 2 enhanced natural killer cell-mediated cytotoxicity in human lymphocyte subpopulations expressing the Leu 7 and Leu 11 antigens.重组白细胞介素2增强了表达Leu 7和Leu 11抗原的人淋巴细胞亚群中自然杀伤细胞介导的细胞毒性。
J Immunol. 1985 Feb;134(2):794-801.
4
Mode of in vitro augmentation of natural killer cell activity by recombinant human interleukin 2: a comparative study of Leu-11+ and Leu-11- cell populations in cord blood and adult peripheral blood.重组人白细胞介素2体外增强自然杀伤细胞活性的方式：脐血和成人外周血中Leu-11 +和Leu-11-细胞群的比较研究
J Immunol. 1985 Oct;135(4):2351-6.
5
The role of interleukin 2 and T11 E rosette antigen in activation and proliferation of human NK clones.白细胞介素2和T11 E玫瑰花结抗原在人自然杀伤细胞克隆激活和增殖中的作用。
J Immunol. 1985 Jul;135(1):672-8.
6
Induction of cytolytic activity by anti-T3 monoclonal antibody. Activation of alloimmune memory cells and natural killer cells from normal and immunodeficient individuals.抗T3单克隆抗体诱导细胞溶解活性。激活来自正常个体和免疫缺陷个体的同种免疫记忆细胞和自然杀伤细胞。
J Clin Invest. 1984 Dec;74(6):2263-71. doi: 10.1172/JCI111653.
7
Induction and blocking of cytolysis in CD2+, CD3- NK and CD2+, CD3+ cytotoxic T lymphocytes via CD2 50 KD sheep erythrocyte receptor.通过CD2 50KD绵羊红细胞受体诱导和阻断CD2⁺、CD3⁻自然杀伤细胞以及CD2⁺、CD3⁺细胞毒性T淋巴细胞中的细胞溶解作用
J Immunol. 1986 Jun 1;136(11):3939-44.
8
Enhancement of natural killer function through activation of the T11 E rosette receptor.通过激活T11 E玫瑰花结受体增强自然杀伤细胞功能。
J Clin Invest. 1987 Jan;79(1):305-8. doi: 10.1172/JCI112800.
9
T lymphocyte adhesion to endothelial cells: mechanisms demonstrated by anti-LFA-1 monoclonal antibodies.T淋巴细胞与内皮细胞的黏附：抗淋巴细胞功能相关抗原-1单克隆抗体所揭示的机制
J Immunol. 1986 Nov 1;137(9):2901-6.
10
Mechanism of cell-mediated cytotoxicity at the single cell level. VI. Direct assessment of the cytotoxic potential of human peripheral blood non-lytic effector-target cell conjugates.单细胞水平的细胞介导细胞毒性机制。VI. 人外周血非裂解效应细胞-靶细胞共轭物细胞毒性潜力的直接评估。
J Immunol. 1984 Feb;132(2):594-8.

引用本文的文献

1
Xenotransplantation: Progress Along Paths Uncertain from Models to Application.异种移植：从模型到应用，在充满不确定性的道路上取得的进展。
ILAR J. 2018 Dec 31;59(3):286-308. doi: 10.1093/ilar/ily015.
2
Adhesion of Epstein-Barr virus-positive natural killer cell lines to cultured endothelial cells stimulated with inflammatory cytokines.爱泼斯坦-巴尔病毒阳性自然杀伤细胞系与经炎性细胞因子刺激的培养内皮细胞的黏附作用。
Clin Exp Immunol. 2008 Mar;151(3):519-27. doi: 10.1111/j.1365-2249.2007.03584.x. Epub 2008 Jan 8.
3
Human single-chain Fv immunoconjugates targeted to a melanoma-associated chondroitin sulfate proteoglycan mediate specific lysis of human melanoma cells by natural killer cells and complement.

本文引用的文献

1
Isolation of human NK cells by density gradient centrifugation.通过密度梯度离心法分离人自然杀伤细胞。
J Immunol Methods. 1980;36(3-4):285-91. doi: 10.1016/0022-1759(80)90133-7.
2
Lymphocyte recognition of lymph node high endothelium. IV. Cell surface structures mediating entry into lymph nodes.淋巴细胞对淋巴结高内皮的识别。IV. 介导进入淋巴结的细胞表面结构。
J Immunol. 1982 Nov;129(5):1911-5.
3
Cells mediating graft rejection in the mouse. I. Lyt-1 cells mediate skin graft rejection.介导小鼠移植物排斥反应的细胞。I. Lyt-1细胞介导皮肤移植物排斥反应。
靶向黑色素瘤相关硫酸软骨素蛋白聚糖的人单链Fv免疫缀合物可介导自然杀伤细胞和补体对人黑色素瘤细胞的特异性裂解。
Proc Natl Acad Sci U S A. 1999 Feb 16;96(4):1627-32. doi: 10.1073/pnas.96.4.1627.
4
An essential role for tumor necrosis factor in natural killer cell-mediated tumor rejection in the peritoneum.肿瘤坏死因子在自然杀伤细胞介导的腹膜肿瘤排斥反应中的重要作用。
J Exp Med. 1998 Nov 2;188(9):1611-9. doi: 10.1084/jem.188.9.1611.
5
Induction of transporter associated with antigen processing by interferon gamma confers endothelial cell cytoprotection against natural killer-mediated lysis.干扰素γ诱导的抗原加工相关转运体赋予内皮细胞针对自然杀伤细胞介导的裂解的细胞保护作用。
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2435-40. doi: 10.1073/pnas.95.5.2435.
6
Effects of 17beta-estradiol on cytokine-induced endothelial cell adhesion molecule expression.17β-雌二醇对细胞因子诱导的内皮细胞黏附分子表达的影响。
J Clin Invest. 1996 Jul 1;98(1):36-42. doi: 10.1172/JCI118774.
7
Quantitative analysis of rod-cored vesicles and dense granules of large granular lymphocytes in the liver, spleen, and peripheral blood of rats.
Cell Tissue Res. 1994 Apr;276(1):187-95. doi: 10.1007/BF00354799.
8
The effects of beta-adrenoceptor stimulation on adhesion of human natural killer cells to cultured endothelium.β-肾上腺素能受体刺激对人自然杀伤细胞与培养内皮细胞黏附的影响。
Br J Pharmacol. 1994 Dec;113(4):1311-6. doi: 10.1111/j.1476-5381.1994.tb17141.x.
9
Lymphocyte adhesion-dependent calcium signaling in human endothelial cells.人内皮细胞中淋巴细胞黏附依赖性钙信号传导
J Cell Biol. 1995 Mar;128(5):969-78. doi: 10.1083/jcb.128.5.969.
10
Heterogeneous activation thresholds to cytokines in genetically distinct endothelial cells: evidence for diverse transcriptional responses.基因不同的内皮细胞对细胞因子的异质性激活阈值：不同转录反应的证据。
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3994-8. doi: 10.1073/pnas.91.9.3994.
J Exp Med. 1981 May 1;153(5):1044-57. doi: 10.1084/jem.153.5.1044.
4
Isolation and growth of endothelial cells from the microvessels of the newborn human foreskin in cell culture.在细胞培养中从新生儿包皮微血管分离内皮细胞并使其生长。
J Invest Dermatol. 1980 Oct;75(4):316-21. doi: 10.1111/1523-1747.ep12530941.
5
Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.与人类T淋巴细胞介导的细胞溶解相关的三种不同抗原：淋巴细胞功能相关抗原-1（LFA-1）、淋巴细胞功能相关抗原-2（LFA-2）和淋巴细胞功能相关抗原-3（LFA-3）。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7489-93. doi: 10.1073/pnas.79.23.7489.
6
A cell-surface molecule involved in organ-specific homing of lymphocytes.一种参与淋巴细胞器官特异性归巢的细胞表面分子。
Nature. 1983;304(5921):30-4. doi: 10.1038/304030a0.
7
NKP-15: a monoclonal antibody reactive against purified human natural killer cells and granulocytes.NKP-15：一种可与纯化的人自然杀伤细胞和粒细胞发生反应的单克隆抗体。
Immunol Lett. 1983 Mar;6(3):143-9. doi: 10.1016/0165-2478(83)90096-2.
8
Which T cells cause graft rejection?哪些T细胞会引发移植排斥反应？
Transplantation. 1982 Mar;33(3):217-21. doi: 10.1097/00007890-198203000-00001.
9
Human large granular lymphocytes are potent producers of interleukin-1.人类大颗粒淋巴细胞是白细胞介素-1的高效生产者。
Nature. 1984;309(5963):56-9. doi: 10.1038/309056a0.
10
The detailed distribution of MHC Class II antigens in normal human organs.MHC II类抗原在正常人体器官中的详细分布。
Transplantation. 1984 Sep;38(3):293-8. doi: 10.1097/00007890-198409000-00019.