仓储害螨嗜卷书虱一种新的39千道尔顿主要变应原的鉴定。

Identification of a new major allergen of 39 kilodaltons of the storage mite Lepidoglyphus destructor.

作者信息

Ansotegui I J, Härfast B, Jeddi-Tehrani M, Johansson E, Johansson S G, van Hage-Hamsten M, Wigzell H

机构信息

Department of Immunology, Karolinska Institute, Karolinska Hospital, Stockholm, Sweden.

出版信息

Immunol Lett. 1991 Feb;27(2):127-30. doi: 10.1016/0165-2478(91)90139-2.

DOI:10.1016/0165-2478(91)90139-2

PMID:2026455

Abstract

The allergen composition of a non-denatured extract of the storage mite Lepidoglyphus destructor was studied by a combination of hybridoma technology, sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) and a sandwich radio-allergosorbent test (four-step RAST). Using hybridoma technology, monoclonal antibodies (mAbs) were generated against the non denatured extract of the L. destructor. After screening by ELISA, mAb 42B6 was selected for further studies. This mAb specifically recognized an antigen of L. destructor of 39 kDa in SDS-PAGE. By a sandwich RAST, sera from 8 patients with known allergy to L. destructor were compared with control sera from 8 allergic patients. The results showed the presence of specific IgE against the 39-kDa protein in the sera of the test group, but not in the control group. There was also a good correlation observed between these data and the results obtained with the classical RAST. These results indicate that the newly identified antigen of 39 kDa is a major allergen of the storage mite Lepidoglyphus destructor.

摘要

通过结合杂交瘤技术、十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和夹心放射变应原吸附试验（四步法RAST），对储藏螨毁灭嗜鳞螨的非变性提取物的变应原成分进行了研究。利用杂交瘤技术，制备了针对毁灭嗜鳞螨非变性提取物的单克隆抗体（mAb）。经酶联免疫吸附测定（ELISA）筛选后，选择单克隆抗体42B6进行进一步研究。该单克隆抗体在SDS-PAGE中能特异性识别一种分子量为39 kDa的毁灭嗜鳞螨抗原。通过夹心RAST，将8例已知对毁灭嗜鳞螨过敏患者的血清与8例过敏患者的对照血清进行了比较。结果显示，试验组血清中存在针对39 kDa蛋白的特异性IgE，而对照组血清中则未检测到。这些数据与经典RAST获得的结果之间也存在良好的相关性。这些结果表明，新鉴定出的39 kDa抗原是储藏螨毁灭嗜鳞螨的主要变应原。