嗜卷书虱与热带博宁螨之间的变应原交叉反应性。

Allergenic crossreactivity between Lepidoglyphus destructor and Blomia tropicalis.

作者信息

Johansson E, Schmidt M, Johansson S G, Machado L, Olsson S, van Hage-Hamsten M

机构信息

Department of Laboratory Medicine, Karolinska Hospital, Stockholm, Sweden.

出版信息

Clin Exp Allergy. 1997 Jun;27(6):691-9.

PMID:9208191

Abstract

BACKGROUND

In general, the non-pyroglyphid mites Lepidoglyphus destructor and Blomia tropicalis show a different geographical distribution. Allergic sensitization to both species have been demonstrated in several investigations. However, whether this reflects cross-reactivity or dual sensitization is so far not known.

OBJECTIVE

The aim of the study was to investigate the allergenicity and allergenic crossreactivity of L. destructor and B. tropicalis using sera from Sweden and Brazil.

METHODS

Allergens in extracts of L. destructor and B. tropicalis were identified with SDS-PAGE and immunoblotting and the crossreactivity was studied by an immunoblot inhibition method. In addition to mite extracts, a recombinant major allergen of L. destructor, Lep d 2, was used.

RESULTS

The extract prepared from L. destructor contained 21 IgE-binding components when using the Swedish or the Brazilian sera. A 15 kDa allergen was recognized by 85% of the Swedish sera and 78% of the Brazilian. The B. tropicalis extract exposed 23 IgE-binding components when the Brazilian sera were used and 19 when the Swedish sera were used. A total of 83% of the Brazilian sera and 80% of the Swedish sera identified a 14.5 kDa allergen. The IgE response of the Swedish serum pool to 10 B. tropicalis allergens was inhibited by L. destructor extract. Likewise, the response of the Brazilian serum pool to four different L. destructor allergens was inhibited by B. tropicalis extract. The recombinant Lep d 2 allergen inhibited 33% of the IgE binding of the Swedish serum pool to the 14.5 kDa allergen in the B. tropicalis extract.

CONCLUSION

Crossreactivity with several proteins from L. destructor and B. tropicalis was demonstrated. The results suggest that a B. tropicalis 14.5 kDa allergen is antigenically crossreactive with recombinant L. destructor allergen Lep d 2.

摘要

背景

一般来说，非疥螨目螨类的食酪螨和热带博氏螨呈现出不同的地理分布。在多项研究中均已证实对这两种螨类均存在过敏致敏现象。然而，这是反映交叉反应性还是双重致敏作用，目前尚不清楚。

目的

本研究旨在利用来自瑞典和巴西的血清，调查食酪螨和热带博氏螨的致敏性及变应原交叉反应性。

方法

采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹法鉴定食酪螨和热带博氏螨提取物中的变应原，并通过免疫印迹抑制法研究交叉反应性。除螨提取物外，还使用了食酪螨的一种重组主要变应原Lep d 2。

结果

使用瑞典或巴西血清时，食酪螨提取物制备物含有21种IgE结合成分。一种15 kDa的变应原被85%的瑞典血清和78%的巴西血清识别。使用巴西血清时，热带博氏螨提取物显示出23种IgE结合成分，使用瑞典血清时为19种。共有83%的巴西血清和80%的瑞典血清识别出一种14.5 kDa的变应原。瑞典混合血清对10种热带博氏螨变应原的IgE反应被食酪螨提取物抑制。同样，巴西混合血清对4种不同食酪螨变应原的反应被热带博氏螨提取物抑制。重组Lep d 2变应原抑制了瑞典混合血清对热带博氏螨提取物中14.5 kDa变应原IgE结合的33%。