Kalish M L, Check I J, Hunter R L
Department of Pathology, Emory University, Atlanta, GA 30322.
J Immunol. 1991 May 15;146(10):3583-90.
The tandem repeat peptide of the circumsporozoite protein of Plasmodium cynomolgi, (NAGG)5, conjugated to BSA or Salmonella flagella, was injected into mice with block copolymer and other adjuvants. The flagella carrier preferentially stimulated IgG2a antibodies to (NAGG)5 that constituted as much as 85% of the total IgG antibody whereas the BSA carrier stimulated as much as 98% IgG1. The distribution of isotypes of antibody to (NAGG)5 was also modified by using the copolymer adjuvants L121 or L141, either alone, or especially in combination with a nontoxic LPS. L121 or L141 increased the proportion of IgG2a and IgG2b antibodies to (NAGG)5 after immunization with (NAGG)5-BSA whereas LPS stimulated further increases in IgG2 antibodies (up to 69% of the total IgG). The hapten density, physical form of emulsion, and route of immunization further affected the isotypes produced in this study.
将食蟹猴疟原虫环子孢子蛋白的串联重复肽（NAGG）5与牛血清白蛋白（BSA）或沙门氏菌鞭毛偶联后，与嵌段共聚物及其他佐剂一起注射到小鼠体内。鞭毛载体优先刺激产生针对（NAGG）5的IgG2a抗体，其在总IgG抗体中所占比例高达85%，而BSA载体刺激产生的IgG1高达98%。使用共聚物佐剂L121或L141单独或尤其与无毒脂多糖联合使用时，针对（NAGG）5的抗体亚型分布也会发生改变。在用（NAGG）5 - BSA免疫后，L121或L141增加了针对（NAGG）5的IgG2a和IgG2b抗体的比例，而脂多糖刺激IgG2抗体进一步增加（高达总IgG的69%）。半抗原密度、乳剂的物理形态以及免疫途径进一步影响了本研究中产生的抗体亚型。
