含8-羟基脱氧鸟苷的DNA的核磁共振研究。
NMR studies of a DNA containing 8-hydroxydeoxyguanosine.
作者信息
Oda Y, Uesugi S, Ikehara M, Nishimura S, Kawase Y, Ishikawa H, Inoue H, Ohtsuka E
机构信息
Faculty of Pharmaceutical Sciences, Osaka University, Japan.
出版信息
Nucleic Acids Res. 1991 Apr 11;19(7):1407-12. doi: 10.1093/nar/19.7.1407.
Abstract
The effects of hydroxylation at the C8 of a deoxyguanosine residue in DNA were studied by NMR analysis of a self-complementary dodecanucleotide, d(C1-G2-C3-oh8G4-A5-A6-T7-T8-C9-G10-C11-G12), which has an 8-hydroxy-2'-deoxyguanosine (oh8dG) residue at the 4th position. NMR data indicate that the 8-hydroxyguanine (oh8G) base takes a 6,8-diketo tautomeric form and is base-paired to C with Watson-Crick type hydrogen bonds in a B-form structure. The thermal stability of the duplex is reduced, but the overall structure is much the same as that of the unmodified d(CGCGAATTCGCG) duplex. The structural changes caused by 8-hydroxylation of the deoxyguanosine, if any, are localized near the modification site.
摘要
通过对一个自互补十二聚体d（C1-G2-C3-oh8G4-A5-A6-T7-T8-C9-G10-C11-G12）进行核磁共振分析，研究了DNA中脱氧鸟苷残基C8位羟基化的影响，该十二聚体在第4位有一个8-羟基-2'-脱氧鸟苷（oh8dG）残基。核磁共振数据表明，8-羟基鸟嘌呤（oh8G）碱基呈6,8-二酮互变异构形式，并在B型结构中通过沃森-克里克型氢键与C碱基配对。双链体的热稳定性降低，但整体结构与未修饰的d（CGCGAATTCGCG）双链体大致相同。脱氧鸟苷8-羟基化引起的结构变化（如果有的话）局限于修饰位点附近。
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