短寡核苷酸片段合成中的简化方法。
Simplications in the synthesis of short oligonucleotide blocks.
作者信息
Broka C, Hozumi T, Arentzen R, Itakura K
出版信息
Nucleic Acids Res. 1980 Nov 25;8(22):5461-71. doi: 10.1093/nar/8.22.5461.
Abstract
A rapid and convenient procedure has been developed for the synthesis of fully protected mono, di and trideoxyribonucleotides utilizing an aryl phosphoroditriazolide. It affords advantages over coupling strategies employing condensing reagents, such as 2,4,6-triisopropylbenzenesulfonyl tetrazolide in preparing small oligonucleotides and is relatively free of the drawbacks inherent in other approaches using bifunctional phosphorylating reagents. In particular, the synthesis of trinucleotide blocks without purification at the dimer stage is described.
摘要
已开发出一种快速便捷的方法，用于利用芳基磷酰三叠氮化物合成完全保护的单、二和三脱氧核糖核苷酸。与使用缩合试剂（如2,4,6-三异丙基苯磺酰四唑）的偶联策略相比，该方法在制备小寡核苷酸方面具有优势，并且相对没有使用双功能磷酸化试剂的其他方法所固有的缺点。特别描述了在二聚体阶段无需纯化即可合成三核苷酸片段的方法。
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本文引用的文献
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J Am Chem Soc. 1975 Dec 10;97(25):7327-32. doi: 10.1021/ja00858a020.
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Chemical synthesis of a self-complementary octanucleotide, dG-G-T-T-A-A-C-C by a modified triester method.采用改良三酯法化学合成自互补八核苷酸dG-G-T-T-A-A-C-C。
Nucleic Acids Res. 1978 Aug;5(8):2809-23. doi: 10.1093/nar/5.8.2809.
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Synthesis of oligonucleotides with sequences identical with or analogous to the 3'-end of 16S ribosomal RNA of Escherichia coli: preparation of A-C-C-U-C-C via the modified phosphotriester method.合成与大肠杆菌16S核糖体RNA 3'-末端序列相同或相似的寡核苷酸：通过改良的磷酸三酯法制备A-C-C-U-C-C。
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