Xiang Y D, Gai J Y, Ma Y H
Soybean Research Institute, Nanjing Agricultural University.
Yi Chuan Xue Bao. 1991;18(1):51-8.
Ten susceptible x resistant and resistant x resistant crosses were made among eight varieties, of which seven were previously identified as resistant to one to four SMV strains, in order to investigate the inheritance of resistance to the four strains (Sa, Sc, Sg, and Sh) and the linkage relationship among the resistant genes. Seeds from F2 individual plants were randomly divided and planted in two separated nurseries, in which the seedlings were inoculated with two different strains. The genotype of each F2 plant was detected from the reaction of its F3 progenies to the two strains. The results showed that the resistance to each of the four strains was conditioned by a dominant gene which was labelled here with Ra, Rc, Rg, and Rh, respectively. All the four loci appeared to be in a same linkage group with the order of Rg--Rh--Ra--Rc. The recombination value between Ra and Rc was estimated to be 25.4 +/- 7.7%, and those between Rg and Rh, Ra and Rh, Ra and Rg, and Rc and Rg to be 27.3 +/- 5.4%, 23.5 +/- 5.2%, 41.1 +/- 8.5%, and 46.5 +/- 7.5%, respectively. The genes resistance to Sa in Kwanggyo and "7222" were determined at the same locus, so were the resistant genes for Sc. In addition, this linkage group seemed to be not related with the first and eighth linkage group.
在八个品种间进行了10次感病×抗病和抗病×抗病杂交，其中七个品种先前已被鉴定对一至四个大豆花叶病毒株系具有抗性，目的是研究对四个株系（Sa、Sc、Sg和Sh）抗性的遗传以及抗性基因间的连锁关系。F2单株的种子被随机分开，种植在两个分开的苗圃中，在苗圃中用两种不同的株系对接种幼苗。根据每个F2植株的F3后代对这两种株系的反应来检测其基因型。结果表明，对四个株系中每个株系的抗性都由一个显性基因控制，这里分别标记为Ra、Rc、Rg和Rh。所有这四个位点似乎位于同一连锁群中，顺序为Rg--Rh--Ra--Rc。估计Ra和Rc之间的重组值为25.4 +/- 7.7%，Rg和Rh、Ra和Rh、Ra和Rg以及Rc和Rg之间的重组值分别为27.3 +/- 5.4%、23.5 +/- 5.2%、41.1 +/- 8.5%和46.5 +/- 7.5%。光野和“7222”中对Sa的抗性基因位于同一基因座，对Sc的抗性基因也是如此。此外，这个连锁群似乎与第一和第八连锁群无关。
