Van Eldik L J, Christie-Pope B, Bolin L M, Shooter E M, Whetsell W O
Department of Pharmacology and Cell Biology, Vanderbilt University, Nashville, TN 37232.
Brain Res. 1991 Mar 1;542(2):280-5. doi: 10.1016/0006-8993(91)91579-p.
We report here that S-100 beta, a protein with neurotrophic activity on central nervous system neurons, stimulates neuritic outgrowth from cultures of dorsal root ganglia (DRG). S-100 beta elicited neurites from explant and dissociated cell cultures of embryonic chick DRG, and the extent of the response varied with the age of the embryo. Specificity was demonstrated by the observation that incubation of S-100 beta with antibodies directed against S-100 beta reduced the neurite outgrowth, whereas incubation of S-100 beta with normal rabbit serum had little effect. S-100 beta also stimulated the area of neuritic outgrowth from organotypic cultures of fetal rat DRG, showing that the activity of the protein is not restricted to a particular species or culture condition. A mutant S-100 beta lacking neurotrophic activity on cerebral cortex neurons was unable to effectively stimulate neurite outgrowth from DRG cultures. These studies suggest that S-100 beta may play a role in neuronal growth and/or maintenance in the peripheral nervous system.
我们在此报告，S-100β是一种对中枢神经系统神经元具有神经营养活性的蛋白质，它能刺激背根神经节（DRG）培养物中的神经突生长。S-100β能诱导胚胎鸡DRG外植体和离散细胞培养物中的神经突生长，且反应程度随胚胎年龄而变化。通过观察发现，将S-100β与抗S-100β抗体一起孵育会减少神经突生长，而将S-100β与正常兔血清一起孵育则几乎没有影响，从而证明了其特异性。S-100β还能刺激胎鼠DRG器官型培养物中神经突生长的面积，表明该蛋白质的活性并不局限于特定物种或培养条件。一种对大脑皮质神经元缺乏神经营养活性的突变型S-100β无法有效刺激DRG培养物中的神经突生长。这些研究表明，S-100β可能在周围神经系统的神经元生长和/或维持中发挥作用。
