Veterinary Public Health Division, Institute for Risk Assessment Sciences, Utrecht University, Yalelaan 2, 3584 CM Utrecht, The Netherlands.
Prev Vet Med. 2010 Jun 1;95(1-2):137-43. doi: 10.1016/j.prevetmed.2010.02.003. Epub 2010 Mar 29.
Salmonella enterica serovar Enteritidis (SE) is an important source of food-related diarrhoea in humans, and table eggs are considered the primordial source of contamination of the human food chain. Using eggs collected at egg-packing stations as samples could be a convenient strategy to detect colonization of layer flocks. The aim of this study was to evaluate egg yolk anti-Salmonella antibody detection using suspension array analysis. An egg yolk panel from contact-infected and non-colonized laying hens was used for the evaluation. Receiver Operating Characteristic (ROC) curves were generated to define a cut-off value and to assess the overall accuracy of the assay. The diagnostic sensitivity and specificity were estimated by maximum likelihood. Sensitivity was quantified on hen level and on sample level, and also quantified as a function of time since colonization. The area under the ROC curve was estimated at 0.984 (se 0.006, P<0.001). Of all colonized contact-infected hens, 67.6% [95% CI: 46.8, 100] developed an antibody response, which was detectable 17.4 days [14.3, 26.9] after colonization. In total, 98% [95.4, 99.4] of the 'immunopositive' hens had test positive eggs. The overall sensitivity of the immunological test was 66.7% [45.9, 98.7] and the specificity was 98.5% [97.8, 99.1]. This study provided essential parameters for optimizing surveillance programs based on detection of antibodies, and indicates that immunology based on examination of egg yolk gives important information about the Salmonella status of the flock.
肠炎沙门氏菌血清型肠炎亚种（SE）是人类食源性腹泻的重要来源，而食用鸡蛋被认为是人类食物链污染的原始来源。使用鸡蛋包装站收集的鸡蛋作为样本可能是检测鸡群定植的一种便捷策略。本研究旨在评估使用悬浮阵列分析检测蛋黄抗沙门氏菌抗体的方法。使用接触感染和未定植的产蛋母鸡的蛋黄面板进行评估。生成接收者操作特征（ROC）曲线以定义截止值并评估该测定的整体准确性。通过最大似然估计来估计诊断的敏感性和特异性。在母鸡水平和样本水平上量化了敏感性，并量化了定植后时间的函数。ROC 曲线下面积估计为 0.984（se 0.006，P<0.001）。在所有定植的接触感染母鸡中，67.6%[95%CI：46.8, 100]发展出抗体反应，在定植后 17.4 天[14.3, 26.9]可检测到。总共，98%[95.4, 99.4]的“免疫阳性”母鸡的鸡蛋检测呈阳性。免疫测试的总体敏感性为 66.7%[45.9, 98.7]，特异性为 98.5%[97.8, 99.1]。本研究为基于抗体检测优化监测计划提供了重要参数，并表明基于蛋黄检查的免疫学为鸡群的沙门氏菌状况提供了重要信息。
