Sachsenweger O, Lohr J E, Kösters J
Staatlichen Tierärztlichen Untersuchungsamt, Aulendorf.
Tierarztl Prax. 1994 Aug;22(4):350-7.
We evaluated three commercial enzyme-linked immunoassay (ELISA) test-kits, using either lipopolysaccharide (LPS) or flagellar extracts as antigens, for the demonstration of Salmonella enteritidis (S. e.) antibodies in serum and egg yolk. They were also compared with conventional serological tests, such as the pullorum rapid slide agglutination test (RST), the pullorum slow agglutination tube test and a rapid slide agglutination test, using gm flagellar extracts as antigen. We tested sera and eggs from six different layer flocks. In 84.1 to 90% of the sera and egg yolks tested the three commercial ELISA test kits gave comparable results. There were a number of serological cross-reaction with other serovars, particularly S. typhimurium. However, the possibility of infections with more than one salmonella serovar, other than S. e., cannot be excluded. The ELISA-antibody levels in serum and egg yolk ran parallel and were still high after one year. However, the level of egg yolk antibodies was on an average 33.9% lower than in serum. The S. e.-ELISA seems to be well suited for epidemiological investigations and for preventive and control measures.
我们评估了三种商用酶联免疫吸附测定（ELISA）试剂盒，它们分别以脂多糖（LPS）或鞭毛提取物作为抗原，用于检测血清和蛋黄中的肠炎沙门氏菌（S. e.）抗体。还将它们与传统血清学检测方法进行了比较，如以gm鞭毛提取物作为抗原的鸡白痢快速玻片凝集试验（RST）、鸡白痢慢速凝集试管试验和快速玻片凝集试验。我们检测了来自六个不同蛋鸡群的血清和鸡蛋。在84.1%至90%的检测血清和蛋黄中，三种商用ELISA试剂盒给出了可比的结果。与其他血清型存在一些血清学交叉反应，特别是鼠伤寒沙门氏菌。然而，不能排除感染除肠炎沙门氏菌之外的一种以上沙门氏菌血清型的可能性。血清和蛋黄中的ELISA抗体水平呈平行关系，且一年后仍保持较高水平。然而，蛋黄抗体水平平均比血清中的低33.9%。肠炎沙门氏菌ELISA似乎非常适合流行病学调查以及预防和控制措施。
