Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Ave., Cleveland OH 44106-4965, USA.
Prog Retin Eye Res. 2010 Sep;29(5):428-42. doi: 10.1016/j.preteyeres.2010.03.002. Epub 2010 Mar 19.
Regeneration of visual pigments is essential for sustained visual function. Although the requirement for non-photochemical regeneration of the visual chromophore, 11-cis-retinal, was recognized early on, it was only recently that the trans to cis retinoid isomerase activity required for this process was assigned to a specific protein, a microsomal membrane enzyme called RPE65. In this review, we outline progress that has been made in the functional characterization of RPE65. We then discuss general concepts related to protein-membrane interactions and the mechanism of the retinoid isomerization reaction and describe some of the important biochemical and structural features of RPE65 with respect to its membrane-binding and enzymatic properties.
视觉色素的再生对于持续的视觉功能至关重要。尽管人们很早就认识到视觉色素 11-顺式视黄醛的非光化学再生需要,但直到最近,人们才将这个过程所需的反式到顺式视黄醛异构酶活性分配到一种特定的蛋白质上,这种蛋白质是一种称为 RPE65 的微粒体膜酶。在这篇综述中,我们概述了在 RPE65 的功能特征方面取得的进展。然后,我们讨论了与蛋白质-膜相互作用以及视黄醛异构化反应机制相关的一般概念,并描述了 RPE65 的一些重要生化和结构特征,涉及到其膜结合和酶活性。