Janczyk Pawel, Hansen Sophie, Bahramsoltani Mahtab, Plendl Johanna
Institute of Veterinary Anatomy, Department of Veterinary Medicine, Freie Universität Berlin, Koserstrasse 20, Berlin, Germany.
J Electron Microsc (Tokyo). 2010;59(4):291-8. doi: 10.1093/jmicro/dfq007. Epub 2010 Mar 18.
This study investigated the morphology and thickness of the glycocalyx linings of microvascular endothelial cells (MVEC). Three distinct cell types were used: the human dermal cells (HDMVEC), the murine cardiac cells (MCMVEC) and the bovine luteal cells (BLMVEC). Cells were cultivated for 48 h. Glycocalyx was stained with ruthenium red and examined under a transmission electron microscope. The glycocalyx of HDMVEC was thin and constant (10-22 nm). No glycocalyx was detected within intracellular vesicles. Two cell populations of MCMVEC were recorded. The minor MCMVEC population was well differentiated and covered with heterogenous glycocalyx (2-200 nm). Conglomerates formed above the baseline along the cell extensions. The major MCMVEC population was undifferentiated and coated by a smooth and thin (12-25 nm) layer of glycocalyx. Intracellular vesicles were also coated with glycocalyx. In the BLMVEC population, 10% had 3-170 nm of discontinuous glycocalyx. Rough conglomerates were observed along cell sprouts. Their intracellular vesicles were coated with glycocalyx. The study found vast differences in the morphology and thickness of endothelial glycocalyx among different MVEC under in vitro cultivation. The only record of active endocytosis was in BLMVEC and MCMVEC. No evidence of active endocytosis was found in HDMVEC.
本研究调查了微血管内皮细胞（MVEC）糖萼衬里的形态和厚度。使用了三种不同的细胞类型：人真皮细胞（HDMVEC）、小鼠心肌细胞（MCMVEC）和牛黄体细胞（BLMVEC）。细胞培养48小时。用钌红对糖萼进行染色，并在透射电子显微镜下检查。HDMVEC的糖萼薄且厚度恒定（10 - 22纳米）。在细胞内小泡中未检测到糖萼。记录到MCMVEC有两个细胞群体。较小的MCMVEC群体分化良好，覆盖有不均一的糖萼（2 - 200纳米）。沿细胞延伸方向在基线以上形成聚集体。较大的MCMVEC群体未分化，被一层光滑且薄（12 - 25纳米）的糖萼覆盖。细胞内小泡也被糖萼覆盖。在BLMVEC群体中，10%具有3 - 170纳米的间断性糖萼。沿细胞芽观察到粗糙的聚集体。它们的细胞内小泡被糖萼覆盖。该研究发现，体外培养条件下不同MVEC的内皮糖萼在形态和厚度上存在巨大差异。活跃内吞作用的唯一记录见于BLMVEC和MCMVEC。在HDMVEC中未发现活跃内吞作用的证据。
