Laboratoire de Virologie, Centre Hospitalier Universitaire de Reims, Service de Microbiologie, Hôpital Robert Debré, Avenue du Général Koenig, 51092 Reims Cedex, France.
J Clin Microbiol. 2010 May;48(5):1943-6. doi: 10.1128/JCM.02181-09. Epub 2010 Mar 19.
From January to December 2007, 973 stool specimens were prospectively collected from children hospitalized for gastroenteritis signs or from neonates and premature cases who were born in two French hospital settings in the north of France. They were tested by rapid enzyme immunoassay (EIA) analyses for rotavirus and adenovirus and by two commercially available ELISA tests for the detection of norovirus and astrovirus. The overall rates of prevalence for rotavirus, norovirus, adenovirus, and astrovirus were 21, 13, 5, and 1.8%, respectively, and they did not significantly differ between the two hospital settings (P=0.12). Mixed virus infections were detected in 32 (3.3%) of the 973 study children and were associated with norovirus in 21 (66%) infants, including 5 premature cases. From fall to spring, norovirus infections accounted for 52% of documented gastroenteritidis viral infections at a time when rotavirus was epidemic, resulting in mixed norovirus and rotavirus gastrointestinal tract infections. Of the 367 documented viral gastroenteritis cases, 15 (4.1%) were identified as nosocomial infections, 5 of which occurred in premature cases. These findings highlight the need to implement norovirus and astrovirus ELISA detection assays in association with rapid EIA rotavirus and adenovirus detection assays for the clinical diagnosis and the nosocomial prevention of gastroenteritis viral infections in pediatric departments.
从 2007 年 1 月至 12 月，前瞻性收集了来自因胃肠炎症状住院的儿童和在法国北部的两家医院出生的新生儿和早产儿的 973 份粪便标本。通过快速酶免疫分析（EIA）分析检测轮状病毒和腺病毒，通过两种市售 ELISA 检测试剂盒检测诺如病毒和星状病毒。轮状病毒、诺如病毒、腺病毒和星状病毒的总流行率分别为 21%、13%、5%和 1.8%，两家医院之间无显著差异（P=0.12）。在 973 例研究儿童中，检测到 32 例（3.3%）混合病毒感染，其中 21 例（66%）婴儿与诺如病毒有关，包括 5 例早产儿。从秋季到春季，诺如病毒感染占轮状病毒流行时记录的病毒性胃肠炎感染的 52%，导致混合性诺如病毒和轮状病毒消化道感染。在 367 例有记录的病毒性胃肠炎病例中，有 15 例（4.1%）被确定为医院感染，其中 5 例发生在早产儿中。这些发现强调需要在快速 EIA 轮状病毒和腺病毒检测的基础上，联合诺如病毒和星状病毒 ELISA 检测试剂盒，对儿科病房的临床诊断和医院感染性胃肠炎病毒的预防。
