Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, ON, M5S 3E5, Canada.
Appl Microbiol Biotechnol. 2010 May;86(6):1903-14. doi: 10.1007/s00253-010-2516-4. Epub 2010 Mar 20.
Proteins secreted by the white-rot, softwood-degrading fungus Phanerochaete carnosa during growth on cellulose and spruce were analyzed using tandem mass spectrometry and de novo sequencing. Homology-driven proteomics was applied to compare P. carnosa peptide sequences to proteins in Phanerochaete chrysosporium using MS BLAST and non-gapped alignment. In this way, 665 and 365 peptides from cellulose and spruce cultivations, respectively, were annotated. Predicted activities included endoglucanases from glycoside hydrolase (GH) families 5, 16, and 61, cellobiohydrolases from GH6 and GH7, GH3 beta-glucosidases, xylanases from GH10 and GH11, GH2 beta-mannosidases, and debranching hemicellulases from GH43 and CE15. Peptides corresponding to glyoxal oxidases, peroxidases, and glycopeptides that could participate in lignin degradation were also detected. Overall, predicted activities detected in extracellular filtrates of cellulose and spruce cultures were similar, suggesting that the adaptation of P. carnosa to growth on lignocellulose might result from fine tuning the expression of similar enzyme families.
使用串联质谱和从头测序分析了白腐菌硬木降解菌 Phanerochaete carnosa 在纤维素和云杉上生长时分泌的蛋白质。同源驱动的蛋白质组学用于将 P. carnosa 肽序列与 Phanerochaete chrysosporium 中的蛋白质进行比较，使用 MS BLAST 和无间隙比对。通过这种方式，分别注释了纤维素和云杉培养物中 665 和 365 个肽。预测的活性包括糖苷水解酶（GH）家族 5、16 和 61 的内切葡聚糖酶、GH6 和 GH7 的纤维二糖水解酶、GH3β-葡萄糖苷酶、GH10 和 GH11 的木聚糖酶、GH2β-甘露糖苷酶和 GH43 和 CE15 的支链半纤维素酶。还检测到了与糖醛氧化酶、过氧化物酶和可能参与木质素降解的糖肽相对应的肽。总的来说，纤维素和云杉培养物细胞外滤液中检测到的预测活性相似，这表明 P. carnosa 对木质纤维素生长的适应可能是通过微调相似酶家族的表达来实现的。
