Jia Dinghong, Wang Bo, Li Xiaolin, Peng Weihong, Zhou Jie, Tan Hao, Tang Jie, Huang Zhongqian, Tan Wei, Gan Bingcheng, Yang Zhirong, Zhao Jian
Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, China.
Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China.
Curr Microbiol. 2017 Aug;74(8):943-951. doi: 10.1007/s00284-017-1268-0. Epub 2017 May 29.
Auricularia polytricha is one of the most widely cultivated edible mushrooms in China. Many advances have been made to A. polytricha, but there is still no proteomic information of this species. Our current understanding was based upon the translated information of its transcriptome or other relative species. This study presented the proteomic information of fruiting-body proteins by shotgun liquid chromatography and tandem mass spectrometry (LC-MS/MS), which identified 15,508 peptides corresponding to 1850 high-confidence proteins. Of these, 1383 were annotated across the GO subcategories with 829 (44.81%) involved in biological process, 908 (49.08%) in molecular function, and 406 (21.95%) in cellular components. Among these high-confidence proteins, 132 proteins were annotated as carbohydrate-active enzymes, of which 51 were secreted enzymes. Moreover, a number of commercially important enzymes were detected, functioning as auxiliary activity (AA) family 5 glyoxal oxidase, AA5 galactose oxidase, glycoside hydrolase (GH) family 20 hexosaminidase, and GH47 alpha-mannosidase. To the best of our knowledge, this is the first study to characterize A. polytricha proteome, and also fills the gap of our knowledge on the under-developed mushroom species.
毛木耳是中国广泛栽培的食用菌之一。对毛木耳已经取得了许多进展，但该物种仍没有蛋白质组学信息。我们目前的了解是基于其转录组或其他相关物种的翻译信息。本研究通过鸟枪法液相色谱和串联质谱（LC-MS/MS）展示了子实体蛋白质的蛋白质组学信息，鉴定出了与1850个高可信度蛋白质相对应的15508个肽段。其中，1383个在GO子类别中被注释，829个（44.81%）参与生物过程，908个（49.08%）参与分子功能，406个（21.95%）参与细胞成分。在这些高可信度蛋白质中，132个被注释为碳水化合物活性酶，其中51个是分泌酶。此外，还检测到了一些具有商业重要性的酶，它们分别作为辅助活性（AA）家族5乙二醛氧化酶、AA5半乳糖氧化酶、糖苷水解酶（GH）家族20氨基己糖苷酶和GH47α-甘露糖苷酶发挥作用。据我们所知，这是第一项对毛木耳蛋白质组进行表征的研究，也填补了我们对这种开发程度较低的蘑菇物种认识上的空白。
