Departamento de Biología Aplicada, Escuela Politécnica Superior, Universidad de Almería, La Cañada de San Urbano, 04120, Almería, Spain.
Fish Physiol Biochem. 2010 Dec;36(4):1079-85. doi: 10.1007/s10695-010-9385-8. Epub 2010 Mar 21.
The present study develops an experimental procedure aimed to estimate the efficiency of protein digestion in fish by measuring both gut transit rate and total amount of the main intestinal proteases (trypsin and chymotrypsin). The selected species was the Nile tilapia (Oreochromis niloticus). Total time for digestion, calculated through the estimation of gut transit rate using differently colored feeds, was 7.15 h. Mean production of trypsin and chymotrypsin was 15.94 and 24.11 mU in the proximal intestine and much lower (2,39, 4.90 mU) in the distal intestine. The enzyme efficiency, calculated from the average enzyme activity and time of residence of the digesta in each intestinal section, points to the major role of proximal intestine in protein digestion for this species. Results are discussed in relation to the main features characterizing digestion in stomachless fish.
本研究开发了一种实验程序，旨在通过测量肠道通过时间和主要肠道蛋白酶（胰蛋白酶和糜蛋白酶）的总量来估计鱼类蛋白质消化的效率。选择的物种是尼罗罗非鱼（Oreochromis niloticus）。通过使用不同颜色的饲料估计肠道通过时间，计算出的总消化时间为 7.15 小时。胰蛋白酶和糜蛋白酶的平均产量分别为 15.94 和 24.11 mU 在近端肠道，而在远端肠道则低得多（2,39, 4.90 mU）。从每个肠道段中消化物的平均酶活性和停留时间计算出的酶效率表明，对于该物种，近端肠道在蛋白质消化中起主要作用。结果与无胃鱼类消化的主要特征进行了讨论。
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