Nogueira Wesclen Vilar, Aznar-García María Jesús, Martínez-Antequera Francisca P, de Las Heras Antonia M Barros, Tesser Marcelo Borges, Garda-Buffon Jaqueline, Moyano Francisco Javier
Escola de Química e Alimentos, Universidade Federal do Rio Grande, Rio Grande 96203-900, RS, Brazil.
Departamento de Biología y Geología, Universidad de Almería, 04120 Almería, Spain.
Animals (Basel). 2023 Sep 28;13(19):3046. doi: 10.3390/ani13193046.
Peroxidase (PO) has been applied in different areas of industrial biotechnology, including the control of contaminants like aflatoxin B in fish feeds. However, its potential negative interactions with the macro and micro components of feeds have not been evaluated. The aim of this study was to evaluate the impact of PO's addition to a feed on compounds like fatty acids and polyphenols using an in vitro simulation of the digestive tract of the tilapia. The influence on fatty acids was determined by changes in the peroxide index, with the feed including PO presenting values four times higher than those of the control feed. On the other hand, the in vitro digestive simulation also evidenced an effect of PO on the bioaccessibility of polyphenols significantly influenced by the total digestion time and temperature. The bioaccessibility of polyphenol ranged from 2.09 to 16.23 μmol of the total Trolox equivalent antioxidant capacity for the combinations evaluated in the study. The greatest bioaccessibility was observed at the central point under the following conditions of digestive hydrolysis: pH of 7, 30 °C, 4.5 h of digestive hydrolysis and an absence of PO.
过氧化物酶（PO）已应用于工业生物技术的不同领域，包括控制鱼饲料中的黄曲霉毒素B等污染物。然而，其与饲料中宏观和微观成分潜在的负面相互作用尚未得到评估。本研究的目的是通过罗非鱼消化道的体外模拟，评估在饲料中添加PO对脂肪酸和多酚等化合物的影响。通过过氧化物指数的变化来确定对脂肪酸的影响，添加PO的饲料其过氧化物指数值比对照饲料高出四倍。另一方面，体外消化模拟也证明了PO对多酚生物可及性有影响，且这种影响受总消化时间和温度的显著影响。在本研究评估的组合中，多酚的生物可及性为每总特洛克斯当量抗氧化能力2.09至16.23微摩尔。在以下消化水解条件下的中心点观察到最大生物可及性：pH为7、30℃、4.5小时消化水解且无PO。
