Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas, Campus Universitário Capão do Leão, s/n, Pelotas, RS, Brazil.
Vet Res Commun. 2010 Mar;34(3):301-6. doi: 10.1007/s11259-010-9354-9. Epub 2010 Mar 23.
The oomycete Pythium insidiosum is the pathogenic cause of pythiosis, a life-threatening disease that affects several animal species. Canines are the second most affected species, and the disease is characterized by the development of cutaneous and gastrointestinal lesions. While concomitant cutaneous and gastrointestinal lesions are rarely found in the same animal, this report documents a case of concurrent cutaneous and gastrointestinal pythiosis in an 18-month-old female Labrador. This dog had an ulcerative cutaneous lesion on the right thoracic region for 12 months that was unresponsive to itraconazole and terbinafine therapy. Two months prior to death and concurrent with the cutaneous lesion, the dog became anorexic with frequent vomiting and bloody stools. At necropsy, a cutaneous lesion that extended subcutaneously into the intercostal muscles was observed. Additionally, the large intestine contained two lesions that caused luminal narrowing. Organs were collected, routinely processed and stained using hematoxylin and eosin and Gomori methenamine silver. Histological examination of the lesions in the large intestine and on the skin revealed areas of necrosis surrounded by a pyogranulomatous infiltrate. Occasionally, black, septate, branching hyphae were detected following staining with Gomori methenamine silver. The diagnosis of pythiosis was confirmed using immunohistochemical methods. This report describes the occurrence of concomitant gastrointestinal and cutaneous lesions in a dog and highlights the therapeutic difficulties encountered with this disease.
卵菌纲中的腐霉属真菌是潜隐菌病的病原体，这种疾病会危及多种动物的生命。犬类是第二大易感染物种，其特征是出现皮肤和胃肠道病变。虽然同时出现皮肤和胃肠道病变在同一动物中很少见，但本报告记录了一例 18 个月大雌性拉布拉多犬同时发生皮肤和胃肠道潜隐菌病的病例。这只狗在右胸区有一个溃疡性皮肤病变，已经持续了 12 个月，对伊曲康唑和特比萘芬治疗没有反应。在死亡前两个月，即出现皮肤病变的同时，狗出现食欲不振、频繁呕吐和带血粪便。在剖检时，观察到一个从皮下延伸到肋间肌的皮肤病变。此外，大肠有两个病变导致管腔变窄。收集了器官，使用苏木精和曙红以及 Gomori 六胺银进行常规处理和染色。对大肠和皮肤病变的组织学检查显示，坏死区域周围有化脓性肉芽肿浸润。偶尔，在使用 Gomori 六胺银染色后会检测到黑色、分隔、分支的菌丝。使用免疫组织化学方法确认了潜隐菌病的诊断。本报告描述了一只狗同时发生胃肠道和皮肤病变的情况，并强调了这种疾病治疗的困难。
