Pharmaceutical Research Center, PCSIR Laboratories Complex, Karachi, Pakistan.
Nat Prod Res. 2010 Mar;24(5):407-15. doi: 10.1080/14786410802696429.
Studies on the aerial parts of Rubus niveus yielded six known compounds, 3,5-dihydroxy benzoic acid C(7)H(6)O(4), (1), gallic acid C(7)H(6)O(5) (2), ethyl galactoside (3), oleanolic acid (4), beta-sitosterol (5) and 3-O-[beta-D-galactopyranosyl-(12)-D-glucopyranoside (6). Besides this, a gallic acid derivative with methyl substitution was synthesised as tetramethyl gallate (3). Together with this derivative, compounds 1, 2, the alcohol soluble, chloroform soluble and petroleum ether soluble extracts of the aerial parts of R. niveus were screened for its nematicidal activity against freshly hatched second stage juveniles of Meloidogyne incognita (root-knot nematode), exhibiting 100, 94, 100, 52, 45 and 14% mortality, respectively of M. incognita after 48 h at 0.5% concentration. Compounds 1, 2 and 3 were found to be more potent than the nematicide Azadirachta indica at the same concentration. Negative results were obtained for nematicidal activity of the petroleum ether extract of R. niveus leaf extract. This is the first report on the isolation of chemical constituents as well as the nematicidal activity of compounds and any part of R. niveus.
对悬钩子属植物地上部分的研究得到了六个已知化合物，3,5-二羟基苯甲酸 C(7)H(6)O(4)，(1)，没食子酸 C(7)H(6)O(5) (2)，乙基半乳糖苷 (3)，齐墩果酸 (4)，β-谷甾醇 (5)和 3-O-[β-D-吡喃半乳糖基-(12)-D-吡喃葡萄糖苷 (6)。除此之外，还合成了一种具有甲基取代的没食子酸衍生物，即四甲基没食子酸酯 (3)。与这种衍生物一起，对悬钩子属植物地上部分的醇溶性、氯仿溶性和石油醚溶性提取物进行了筛选，以研究其对刚孵化的第二阶段幼虫的杀线虫活性，在 0.5%浓度下，48 小时后，对根结线虫的死亡率分别为 100%、94%、100%、52%、45%和 14%。化合物 1、2 和 3 的杀线虫活性比同样浓度的印楝素更强。悬钩子属植物叶石油醚提取物的杀线虫活性呈阴性。这是首次报道悬钩子属植物化学成分的分离以及化合物和悬钩子属植物任何部分的杀线虫活性。
