Research and Development Division, Streck Inc., La Vista, NE 68128, USA.
Prenat Diagn. 2010 May;30(5):418-24. doi: 10.1002/pd.2484.
To develop a standardized blood collection device that preserves fetal cell-free DNA and minimizes the cell-free DNA background in maternal plasma.
Blood samples were drawn from healthy pregnant donors into K(3)EDTA (BD vacutainer) and Cell-free DNA BCT tubes (Streck, Inc.) and kept at ambient temperature. Plasma was separated by centrifugation and cell-free DNA was extracted. Cell-free DNA from plasma was quantified by quantitative real-time polymerase chain reaction.
Blood drawn into Cell-free DNA BCT tubes showed no change in the original proportion of fetal cell-free DNA during a 14-day storage period at ambient temperature. Conversely, maternal blood drawn into K(3)EDTA tubes showed a steady reduction in the original proportion of fetal cell-free DNA over the same time period. Using maternal plasma stored in Cell-free DNA BCT tubes for 14 days, fetal cell-free DNA was amplified 80-fold using whole genome amplification (WGA).
Using Streck's Cell-free DNA BCT tubes, it is possible to preserve the original proportion of fetal cell-free DNA for extended times as well as minimize the post-sampling maternal cell-free DNA background. Preserved in this way, fetal cell-free DNA can be amplified by WGA technology to be used in prenatal diagnostic tests.
开发一种标准化的血液采集装置，以保存胎儿游离 DNA 并最大限度地减少母体外周血中游离 DNA 的背景。
将健康孕妇的血液样本分别采集到 K(3)EDTA（BD vacutainer）和游离 DNA BCT 管（Streck，Inc.）中，并在室温下保存。通过离心分离血浆并提取游离 DNA。通过实时定量聚合酶链反应定量游离 DNA。
在室温下储存 14 天期间，Cell-free DNA BCT 管中采集的血液中胎儿游离 DNA 的原始比例没有变化。相比之下，在同一时间内，K(3)EDTA 管中采集的母血中胎儿游离 DNA 的原始比例持续下降。使用在 Cell-free DNA BCT 管中储存 14 天的母体外周血，使用全基因组扩增（WGA）可将胎儿游离 DNA 扩增 80 倍。
使用 Streck 的 Cell-free DNA BCT 管，不仅可以长时间保持胎儿游离 DNA 的原始比例，还可以最大限度地减少采样后母体外周血中游离 DNA 的背景。以这种方式保存的胎儿游离 DNA 可以通过 WGA 技术扩增，用于产前诊断测试。
