Ma Li, Zheng Li Wu, Sham Mai Har, Cheung Lim Kwong
Discipline of Oral & Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.
J Oral Maxillofac Surg. 2010 Apr;68(4):777-81. doi: 10.1016/j.joms.2009.07.102.
This study aims to evaluate the influence of nicotine on the gene expression of osteogenic and angiogenic factors in bone regeneration by use of a nicotine-compromised rabbit model of mandibular lengthening.
Thirty adult New Zealand white rabbits were randomly assigned to the nicotine group or the control group. The total nicotine or placebo exposure time for all animals was 7 weeks. Unilateral mandibular distraction osteogenesis was performed. Five animals in each group were sacrificed at day 5, day 11, and day 18, respectively, after commencement of active distraction. The distraction regenerate samples were harvested, and the messenger ribonucleic acid expression of bone transforming growth factor beta(1), platelet-derived growth factor A, and basic fibroblast growth factor was assayed by real-time polymerase chain reaction analysis.
The messenger ribonucleic acid expression of transforming growth factor beta(1), platelet-derived growth factor A, and basic fibroblast growth factor was significantly inhibited by nicotine exposure at a variety of time points.
The presence of nicotine inhibited the gene expression of angiogenic and osteogenic factors resulting in compromised bone regeneration.
本研究旨在通过使用尼古丁损害的下颌骨延长兔模型，评估尼古丁对骨再生中骨生成和血管生成因子基因表达的影响。
30只成年新西兰白兔随机分为尼古丁组和对照组。所有动物的总尼古丁或安慰剂暴露时间为7周。进行单侧下颌骨牵张成骨。在开始主动牵张后，每组分别于第5天、第11天和第18天处死5只动物。采集牵张再生样本，通过实时聚合酶链反应分析检测骨转化生长因子β（1）、血小板衍生生长因子A和碱性成纤维细胞生长因子的信使核糖核酸表达。
在多个时间点，尼古丁暴露显著抑制了转化生长因子β（1）、血小板衍生生长因子A和碱性成纤维细胞生长因子的信使核糖核酸表达。
尼古丁的存在抑制了血管生成和骨生成因子的基因表达，导致骨再生受损。
