Dalian Institute of Chemical Physics, CAS, Dalian 116023, China.
Bioresour Technol. 2010 Aug;101(15):6124-9. doi: 10.1016/j.biortech.2010.02.111. Epub 2010 Mar 21.
Nitrogen-limited conditions have been routinely prepared for efficient lipid production by oleaginous microorganisms. However, it is difficult to attain high cellular lipid contents with natural nitrogen-rich substrates. In the present study, we showed that lipid accumulation by Rhodosporidium toruloides Y4 was directly linked to the carbon to phosphorus (C/P) molar ratios of the culture media. Moreover, such lipid accumulation phenomena were effective regardless of the presence of high amounts of nitrogen sources. Thus, cellular lipid content and lipid yield were 62.2% and 0.205 g/g glucose, respectively, using a medium with a carbon to nitrogen (C/N) molar ratio of 6.1 and a C/P molar ratio of 9552. This work suggested that phosphorus limitation can be equally effective and efficient to mediate lipid accumulation, which in turn, provides opportunities to produce microbial lipid more economically using natural or waste materials with high nitrogen content.
在进行油脂生物合成时，通常采用氮限制条件来提高油脂产量。然而，利用天然富氮底物很难达到高细胞油脂含量。在本研究中，我们发现粘红酵母 Y4 的油脂积累与培养基中的碳磷（C/P）摩尔比直接相关。此外，无论是否存在大量氮源，这种油脂积累现象都是有效的。因此，在 C/N 摩尔比为 6.1，C/P 摩尔比为 9552 的培养基中，细胞油脂含量和油脂产率分别达到 62.2%和 0.205 g/g 葡萄糖。本研究表明，磷限制同样可以有效地介导油脂积累，这为利用含氮量高的天然或废物原料更经济地生产微生物油脂提供了机会。
