School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, PR China; Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, Dalian 116023, PR China.
J Biotechnol. 2013 Oct 10;168(1):85-9. doi: 10.1016/j.jbiotec.2013.08.010. Epub 2013 Aug 18.
Microbial lipids are potential alternative feedstock for biofuel and oleochemical industries. The oleaginous yeast Rhodosporidium toruloides AS 2.1389 is an excellent lipid producer. To attain parameters for the understanding of the lipid production process, we performed continuous cultivation experiments under either carbon or nitrogen limitation. The maintenance coefficient and maximum cell mass yield for this yeast were determined as 5.7 mg glucose/g cell/h and 0.42 g cell/g glucose, respectively, under carbon limitation. Under nitrogen limitation, the highest lipid yield of 0.19 g/g was observed at the dilution rate of 0.02 h(-1) while the highest specific lipid formation rate of 0.058 g/g cell/h at the dilution rate of 0.08 h(-1). A kinetic model of lipid formation under steady state conditions was developed, parameters estimated, and optimal continuous cultivation conditions forecasted. These data should be very helpful to develop and design more efficient bioprocesses for microbial lipid production.
微生物油脂是生物燃料和油脂化工行业有潜力的替代原料。产油酵母粘红酵母 AS 2.1389 是一种出色的油脂生产菌。为了获得对油脂生产过程的理解，我们在碳源或氮源限制条件下进行了连续培养实验。在碳源限制条件下，该酵母的维持系数和最大细胞质量产率分别为 5.7mg 葡萄糖/g 细胞/h 和 0.42g 细胞/g 葡萄糖。在氮源限制条件下，在稀释率为 0.02h(-1)时观察到最高的 0.19g/g 油脂产量，而在稀释率为 0.08h(-1)时观察到最高的 0.058g/g 细胞/h 的比脂形成速率。建立了稳态条件下脂形成的动力学模型，估算了参数，并预测了最佳的连续培养条件。这些数据应该对开发和设计更有效的微生物油脂生产生物工艺非常有帮助。
