Rusk D, Sutton G P, Look K Y, Roman A
Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis.
Obstet Gynecol. 1991 Jun;77(6):918-22.
We have analyzed a number of invasive squamous cell carcinomas for the presence of human papillomavirus (HPV) DNA using dot blot and Southern blot analysis. Seven of 31 samples (23%) were positive by dot blot and/or Southern blot analysis. In contrast, six of 11 (55%) of vulvar intraepithelial neoplasias contained HPV DNA by dot blot and/or Southern blot hybridization. Less than 50% of the invasive vulvar carcinomas contained detectable HPV DNA. The average age at onset of vulvar carcinoma is higher than that for cervical carcinoma (in which HPV DNA is detected in over 80% of cases). Therefore, the role of HPV in the genesis of vulvar carcinoma may be different from the role of HPV in the genesis of cervical carcinoma.
我们使用斑点印迹法和Southern印迹法分析了许多浸润性鳞状细胞癌，以检测人乳头瘤病毒（HPV）DNA的存在情况。在31个样本中，有7个（23%）通过斑点印迹法和/或Southern印迹法分析呈阳性。相比之下，在11例外阴上皮内瘤变中，有6个（55%）通过斑点印迹法和/或Southern印迹杂交检测到HPV DNA。不到50%的浸润性外阴癌含有可检测到的HPV DNA。外阴癌发病的平均年龄高于宫颈癌（宫颈癌病例中超过80%可检测到HPV DNA）。因此，HPV在外阴癌发生中的作用可能与HPV在宫颈癌发生中的作用不同。
