Carson L F, Twiggs L B, Okagaki T, Clark B A, Ostrow R S, Faras A J
Department of Obstetrics and Gynecology, University of Minnesota, Minneapolis.
Obstet Gynecol. 1988 Jul;72(1):63-7.
The tissues from 16 cases of adenosquamous carcinoma (pseudoglandular squamous cell carcinoma or adenoacanthoma of the sweat glands of Lever) and 26 cases of invasive squamous cell carcinoma of the vulva were studied for the presence of human papillomavirus (HPV) genomes using Southern blot hybridization on fresh tissues. Types 1, 2, 3, 4, 5, 6, 16, and 18 HPV DNA probes and in situ hybridization were used on formalin-fixed paraffin sections using type 2, 6, 16, and 18 HPV DNA probes. Only one case of adenosquamous carcinoma contained an undetermined type of HPV DNA, whereas five cases of squamous cell carcinoma contained HPV DNA. Three of these five cases contained type 16, one type 6 HPV, and two an undetermined type. These results demonstrate HPV DNA associations with malignancy of the vulva that are similar to those observed elsewhere in the genital tract.
采用新鲜组织Southern印迹杂交技术，对16例腺鳞癌（假腺性鳞状细胞癌或Lever汗腺腺棘皮瘤）及26例外阴浸润性鳞状细胞癌组织进行人乳头瘤病毒（HPV）基因组检测。使用1、2、3、4、5、6、16和18型HPV DNA探针，对经福尔马林固定的石蜡切片采用2、6、16和18型HPV DNA探针进行原位杂交。仅1例腺鳞癌含有未确定类型的HPV DNA，而5例鳞状细胞癌含有HPV DNA。这5例中3例含有16型，1例含有6型HPV，2例为未确定类型。这些结果表明，HPV DNA与外阴恶性肿瘤的关联与在生殖道其他部位观察到的相似。
