二级脉冲场凝胶电泳：一种更快分离更大DNA分子的新方法。

Zhang T Y, Smith C L, Cantor C R

Human Genome Center, Lawrence Berkeley Laboratory, University of California, Berkeley 94720.

Nucleic Acids Res. 1991 Mar 25;19(6):1291-6. doi: 10.1093/nar/19.6.1291.

A novel technique, which we call secondary pulsed field gel electrophoresis (SPFG) has been developed. In SPFG, short pulses are applied in the direction of net migration of the DNA in addition to the reorienting pulses used in conventional pulsed field electrophoresis (PFG). Experimental results show that SPFG extends and improves the electrophoretic resolution of DNA for molecules from 0.5 megabase pairs to over 10 megabase pairs in size. This improved resolution is obtained with dramatically shorter run times. Thus SPFG appears to circumvent a number of the key limitations in previous PFG protocols.

我们开发了一种名为二次脉冲场凝胶电泳（SPFG）的新技术。在SPFG中，除了传统脉冲场电泳（PFG）中使用的重新定向脉冲外，还会在DNA净迁移方向上施加短脉冲。实验结果表明，对于大小从0.5兆碱基对到超过10兆碱基对的分子，SPFG扩展并提高了DNA的电泳分辨率。这种提高的分辨率是在显著更短的运行时间内获得的。因此，SPFG似乎规避了先前PFG方案中的一些关键限制。