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Molecular genetic analysis of DNA obtained from fixed, air dried or paraffin embedded sources.

作者信息

Grünewald K, Lyons J, Hansen-Hagge T E, Janssen J W, Feichtinger H, Bartram C R

机构信息

Department of Internal Medicine, University of Innsbruck, Austria.

出版信息

Ann Hematol. 1991 Apr;62(4):108-14. doi: 10.1007/BF01702923.

DOI:10.1007/BF01702923
PMID:2031973
Abstract

In the present study methods are described for the analysis of the genomic structure of DNA isolated from tissues that have been stored up to several years as air-dried or stained bone marrow smears, bone marrow biopsies in "Histicon", chromosomal preparations in fixative or as formalin fixed tissues embedded in paraffin. By application of the new techniques clonal B- and T-cell disorders, Philadelphia chromosome translocations and ras oncogene mutations in pancreatic carcinomas could be detected. Thus, these DNA extraction procedures may open new avenues to pathological archives and enable the analysis of samples when fresh material is not available.

摘要

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Biochem Biophys Res Commun. 1987 Feb 13;142(3):710-6. doi: 10.1016/0006-291x(87)91472-0.