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来自固定细胞遗传学标本的高分子量DNA。

High molecular weight DNA from fixed cytogenetic preparations.

作者信息

Barker P E, Testa J R, Parsa N Z, Snyder R

出版信息

Am J Hum Genet. 1986 Nov;39(5):661-8.

PMID:3788978
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1684056/
Abstract

Cell pellets that have been stored in routine clinical cytogenetic fixative were studied for the presence of intact DNA. A method for the isolation of high molecular weight DNA from fixed cytogenetic preparations of human leukocytes, bone marrow, and cell hybrid cultures is presented. DNA preparations from fixed pellets were cleaved with restriction enzymes, transferred to nitrocellulose filters after agarose gel electrophoresis, and hybridized to radiolabeled probes to demonstrate that fixed cell pellets could yield DNA of sufficient quality for Southern blot hybridization analysis. This protocol may be useful for molecular analysis of DNA from fixed cell pellets of patients who are unavailable for additional sampling.

摘要

研究了储存在常规临床细胞遗传学固定剂中的细胞沉淀，以检测完整DNA的存在情况。本文介绍了一种从人白细胞、骨髓和细胞杂交培养物的固定细胞遗传学标本中分离高分子量DNA的方法。固定沉淀中的DNA制备物用限制性内切酶切割，经琼脂糖凝胶电泳后转移至硝酸纤维素滤膜上，并与放射性标记探针杂交，以证明固定细胞沉淀能够产生质量足以用于Southern印迹杂交分析的DNA。该方案对于无法进行额外采样的患者的固定细胞沉淀中的DNA分子分析可能有用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/872b/1684056/c2a653d66d3f/ajhg00147-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/872b/1684056/c3d961ff8814/ajhg00147-0106-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/872b/1684056/3c8f651f4425/ajhg00147-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/872b/1684056/f2e2bceae050/ajhg00147-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/872b/1684056/cd312f976ce4/ajhg00147-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/872b/1684056/c2a653d66d3f/ajhg00147-0110-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/872b/1684056/c3d961ff8814/ajhg00147-0106-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/872b/1684056/3c8f651f4425/ajhg00147-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/872b/1684056/f2e2bceae050/ajhg00147-0108-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/872b/1684056/cd312f976ce4/ajhg00147-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/872b/1684056/c2a653d66d3f/ajhg00147-0110-a.jpg

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High molecular weight DNA from fixed cytogenetic preparations.来自固定细胞遗传学标本的高分子量DNA。
Am J Hum Genet. 1986 Nov;39(5):661-8.
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引用本文的文献

1
CGH and SNP array using DNA extracted from fixed cytogenetic preparations and long-term refrigerated bone marrow specimens.使用从固定细胞遗传学标本和长期冷藏骨髓标本中提取的DNA进行比较基因组杂交（CGH）和单核苷酸多态性（SNP）阵列分析。
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2
Diagnosis of DiGeorge syndrome in nuclei released from archival autoptic heart specimens using fluorescence in situ hybridization.利用荧光原位杂交技术对存档尸检心脏标本中释放的细胞核进行DiGeorge综合征的诊断。
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Efficient reidentification of chromosome slides from human metaphase spreads via microsatellite polymerase chain reaction.

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