de Vaux Saint-Cyr C, Loisillier F, Zuinghedau J
Ann Microbiol (Paris). 1977 Oct;128(3):385-98.
Hamster fibroblasts transformed in vivo by the SV40 virus (TSV5Cl2) induce tumours when injected into adult hamsters and antibodies present in sera of tumour-bearing animals are used to demonstrate the presence of the antigens specific for the viral transformation. These tumours are a very useful tool in studying the evolution of the immune response during the tumour growth. A systematic histological examination of the spleen, thymus and lymph nodes was undertaken and the results thus obtained were correlated with a parallel study of the cells of the peritumoral region, the thymus and the spleen by immunofluorescence using antisera of different specificities. We were able to show that the plasma cells which make up the early peritumoral reaction and the lymphoblasts found later in spleen and thymus both synthesize antibodies directed against virus induced antigens. Circulating antibody directed against the same antigens is first detected at/or about the time that the peritumoral plasma cell reaction disappears and increases progressively as tumour weight increases.
被SV40病毒（TSV5Cl2）在体内转化的仓鼠成纤维细胞，注射到成年仓鼠体内时会诱发肿瘤，利用荷瘤动物血清中存在的抗体来证明病毒转化特异性抗原的存在。这些肿瘤是研究肿瘤生长过程中免疫反应演变的非常有用的工具。对脾脏、胸腺和淋巴结进行了系统的组织学检查，并将所得结果与使用不同特异性抗血清通过免疫荧光对肿瘤周围区域、胸腺和脾脏细胞进行的平行研究相关联。我们能够证明，构成早期肿瘤周围反应的浆细胞以及后来在脾脏和胸腺中发现的淋巴母细胞都合成针对病毒诱导抗原的抗体。针对相同抗原的循环抗体首先在肿瘤周围浆细胞反应消失时或大约此时被检测到，并随着肿瘤重量的增加而逐渐增加。
