Weber J A, van Zanten A P
Department of Clinical Chemistry, Slotervaart Hospital, Amsterdam, The Netherlands.
Clin Chem. 1991 May;37(5):695-700.
We measured the interference of carbonyl compounds, drugs, and other substances in human serum on the determination of creatinine by the two-point, fixed-time kinetic modification of the Jaffé reaction as well as by four enzymatic methods. We added known concentrations of the interfering substances to a solution of creatinine in water. For bilirubin, we used both pooled normal sera with added bilirubin and icteric patient sera. The magnitude of interferences varies widely from method to method. Carbonyl compounds, dopamine, cephalosporines, and bilirubin interfere with the Jaffé reaction. Bilirubin, creatine, dopamine, ascorbic acid, and sarcosine interfere with the enzymatic methods. We conclude that the elimination of interferences in the determination of creatinine has still not been achieved.
我们测定了人血清中的羰基化合物、药物及其他物质对采用改良两点固定时间动力学法的Jaffe反应以及四种酶法测定肌酐的干扰。我们将已知浓度的干扰物质加入到水中的肌酐溶液中。对于胆红素，我们使用添加了胆红素的混合正常血清和黄疸患者血清。干扰程度因方法不同而有很大差异。羰基化合物、多巴胺、头孢菌素和胆红素会干扰Jaffe反应。胆红素、肌酸、多巴胺、抗坏血酸和肌氨酸会干扰酶法。我们得出结论，在肌酐测定中消除干扰仍未实现。
