Standefer J C, Backer R C
Department of Pathology, University of New Mexico School of Medicine, Albuquerque 87131.
Clin Chem. 1991 May;37(5):733-8.
We investigated the precision, linearity, accuracy, and stability of quantitative results for five drugs of abuse [amphetamines, benzoylecgonine, opiates, phencyclidine, and the cannabinoid-tetrahydrocannabinol (THC)-9-acid metabolite], analyzed in control specimens by using EMIT d.a.u. reagents (Syva Co.) with a Monarch 2000 analyzer with a nonlinear interpolation curve-fitting algorithm. The within-day and between-days coefficients of variation (CVs) were less than 5% for all drugs except THC-9-acid, which had a CV between 10% and 20%. The drift of control values during a 30-day stability study was less than 10% from target values for three weeks after a single calibration, except for THC-9-acid control values, which were stable for only two to three days. Daily calibration reduced the drift away from target values during the 30-day stability study and produced optimum precision of all drug assays. Mean control values near the National Institute on Drug Abuse cutoff limits were within 10% of their target values.
我们使用EMIT d.a.u.试剂（Syva公司）和带有非线性插值曲线拟合算法的Monarch 2000分析仪，对对照样本中分析的五种滥用药物[苯丙胺、苯甲酰芽子碱、阿片类药物、苯环利定和大麻素 - 四氢大麻酚（THC）-9-酸代谢物]的定量结果的精密度、线性、准确性和稳定性进行了研究。除THC-9-酸外，所有药物的日内和日间变异系数（CV）均小于5%，THC-9-酸的CV在10%至20%之间。在30天稳定性研究期间，单次校准后三周内，除THC-9-酸对照值仅稳定两到三天外，对照值相对于目标值的漂移小于10%。每日校准减少了30天稳定性研究期间偏离目标值的漂移，并使所有药物检测达到最佳精密度。接近美国国家药物滥用研究所临界值的平均对照值在其目标值的10%以内。
