Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, P. R. China.
J Insect Sci. 2008;8:1-7. doi: 10.1673/031.008.5501.
A novel cDNA clone encoding a cytochrome P450 gene has been isolated from the insecticide-susceptible strain of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae). The nucleotide sequence of the clone, designated CYP345D3, was determined. The cDNA is 1554 bp in length and contains an open reading frame from base pairs 32 to 1513, encoding a protein of 493 amino acid residues and a predicted molecular weight of 57466 Daltons. The putative protein contains the classic heme-binding sequence motif FxxGxxxCxG (residues 430-439) conserved among all P450 enzymes as well as other characteristic motifs of the cytochrome P450s. Comparison of the deduced amino acid sequence with other CYP members shows that CYP345D3 shares 91% identity with the previously published sequence of CYP345D1 from the T. castaneum genome project and the nucleotide sequence identity between them is less than 80%. Phylogenetic analysis of amino acid sequences from members of various P450 families indicated close phylogenetic relationship of CYP345D3 with CYP6 of other insects than those from mammals and amore distant relationship to P450 from other families. CYP345D3 was submitted to GenBank, accession number EU008544.
已从杀虫剂敏感型赤拟谷盗（Tribolium castaneum）（鞘翅目：拟步甲科）中分离出编码细胞色素 P450 基因的新 cDNA 克隆。该克隆的核苷酸序列被命名为 CYP345D3。cDNA 长 1554bp，包含从碱基 32 到 1513 的开放阅读框，编码 493 个氨基酸残基的蛋白质和预测分子量为 57466 道尔顿的蛋白质。假定的蛋白质包含经典的血红素结合序列基序 FxxGxxxCxG（残基 430-439），该基序在所有 P450 酶中保守，以及细胞色素 P450 的其他特征基序。与其他 CYP 成员推导的氨基酸序列比较表明，CYP345D3 与之前发表的 T. castaneum 基因组项目的 CYP345D1 序列具有 91%的同一性，它们之间的核苷酸序列同一性小于 80%。来自各种 P450 家族成员的氨基酸序列的系统发育分析表明，CYP345D3 与其他昆虫的 CYP6 具有密切的系统发育关系，而与哺乳动物和其他家族的 P450 关系较远。CYP345D3 已提交给 GenBank，登录号为 EU008544。
