Liang Xiao, Xiao Da, He Yanping, Yao Jianxiu, Zhu Guonian, Zhu Kun Yan
Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China.
Department of Entomology, 123 Waters Hall, Kansas State University, Manhattan, KS 66506, USA.
Int J Mol Sci. 2015 Jan 19;16(1):2078-98. doi: 10.3390/ijms16012078.
Some cytochrome P450 (CYP) genes are known for their rapid up-regulation in response to insecticide exposures in insects. To date, however, limited information is available with respect to the relationships among the insecticide type, insecticide concentration, exposure duration and the up-regulated CYP genes. In this study, we examined the transcriptional response of eight selected CYP genes, including CYP4G7, CYP4Q4, CYP4BR3, CYP12H1, CYP6BK11, CYP9D4, CYP9Z5 and CYP345A1, to each of four insecticides in the red flour beetle, Tribolium castaneum. Reverse transcription quantitative PCR (RT-qPCR) revealed that CYP4G7 and CYP345A1 can be significantly up-regulated by cypermethrin (1.97- and 2.06-fold, respectively), permethrin (2.00- and 2.03-fold) and lambda-cyhalothrin (1.73- and 1.81-fold), whereas CYP4BR3 and CYP345A1 can be significantly up-regulated by imidacloprid (1.99- and 1.83-fold) when 20-day larvae were exposed to each of these insecticides at the concentration of LC20 for 24 h. Our studies also showed that similar levels of up-regulation can be achieved for CYP4G7, CYP4BR3 and CYP345A1 by cypermethrin, permethrin, lambda-cyhalothrin or imidacloprid with approximately one fourth of LC20 in 6 h. Our study demonstrated that up-regulation of these CYP genes was rapid and only required low concentrations of insecticides, and the up-regulation not only depended on the CYP genes but also the type of insecticides. Our results along with those from previous studies also indicated that there were no specific patterns for predicting the up-regulation of specific CYP gene families based on the insecticide classification.
一些细胞色素P450（CYP）基因因在昆虫接触杀虫剂后迅速上调而闻名。然而，迄今为止，关于杀虫剂类型、杀虫剂浓度、暴露持续时间与上调的CYP基因之间的关系，可用信息有限。在本研究中，我们检测了红粉甲虫（Tribolium castaneum）中八个选定的CYP基因，包括CYP4G7、CYP4Q4、CYP4BR3、CYP12H1、CYP6BK11、CYP9D4、CYP9Z5和CYP345A1，对四种杀虫剂中每一种的转录反应。逆转录定量PCR（RT-qPCR）显示，当20日龄幼虫在LC20浓度下暴露于氯氰菊酯、氯菊酯和高效氯氟氰菊酯24小时时，CYP4G7和CYP345A1可分别显著上调1.97倍和2.06倍、2.00倍和2.03倍、1.73倍和1.81倍；而当20日龄幼虫在LC20浓度下暴露于吡虫啉24小时时，CYP4BR3和CYP345A1可分别显著上调1.99倍和1.83倍。我们的研究还表明，氯氰菊酯、氯菊酯、高效氯氟氰菊酯或吡虫啉在6小时内以约四分之一的LC20浓度可使CYP4G7、CYP4BR3和CYP345A1达到相似的上调水平。我们的研究表明，这些CYP基因上调迅速，仅需低浓度杀虫剂，且上调不仅取决于CYP基因，还取决于杀虫剂类型。我们的结果以及先前研究的结果还表明，基于杀虫剂分类预测特定CYP基因家族的上调没有特定模式。
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