Animal Genomics Bioinformatics Division, National Institute of Animal Science, Suwon, 441-701, Republic of Korea.
The Ohio State University, Department of Animal Science, Columbus OH 43210, USA.
Meat Sci. 2015 Jul;105:57-62. doi: 10.1016/j.meatsci.2015.02.014. Epub 2015 Mar 3.
This study aimed to verify genetic effects of the bovine adiponectin (ADIPOQ) gene on carcass traits of Hanwoo cattle. The measured carcass traits were marbling score (MAR), backfat thickness (BFT), loineye area (LEA), and carcass weight (CAW). Selection of primers was based on the bovine ADIPOQ sequence, and the analysis amplified approximately 267 and 333 bp genomic segments, including 67 bp of insertions in the promoter region. Sequencing analysis confirmed genetic variants (g.81966235C>T, g.81966377T>C, and g.81966364D>I) that showed significant effects on MAR. The present results suggest that the identified SNPs are useful genetic markers for the improvement of carcass traits in Hanwoo cattle.
本研究旨在验证牛脂联素（ADIPOQ）基因对韩牛胴体性状的遗传效应。所测胴体性状包括大理石花纹评分（MAR）、背膘厚度（BFT）、腰眼面积（LEA）和胴体重（CAW）。引物的选择基于牛 ADIPOQ 序列，分析扩增了大约 267 和 333 bp 的基因组片段，包括启动子区域 67 bp 的插入。测序分析证实了对 MAR 有显著影响的遗传变异（g.81966235C>T、g.81966377T>C 和 g.81966364D>I）。本研究结果表明，所鉴定的 SNP 是改良韩牛胴体性状的有用遗传标记。
