Key Laboratory of Arid and Grassland Agroecology, Ministry of Education, School of Life Science, Lanzhou University, Lanzhou 730000, PR China.
Biochem Biophys Res Commun. 2010 May 28;396(2):187-92. doi: 10.1016/j.bbrc.2010.03.119. Epub 2010 Mar 21.
DNA methyltransferase2 (DNMT2) is always deemed to be enigmatic, because it contains highly conserved DNA methyltransferase motifs but lacks the DNA methylation catalytic capability. Here we show that Arabidopsis DNA methyltransferase2 (AtDNMT2) is localized in nucleus and associates with histone deacetylation. Bimolecular fluorescence complementation and pull-down assays show AtDNMT2 interacts with type-2 histone deacetylases (AtHD2s), a unique type of histone deacetylase family in plants. Through analyzing the expression of AtDNMT2: ss-glucuronidase (GUS) fusion protein, we demonstrate that AtDNMT2 has the ability to repress gene expression at transcription level. Meanwhile, the expression of AtDNMT2 gene is altered in athd2c mutant plants. We propose that AtDNMT2 possibly involves in the activity of histone deacetylation and plant epigenetic regulatory network.
DNA 甲基转移酶 2（DNMT2）一直被认为是神秘的，因为它含有高度保守的 DNA 甲基转移酶基序，但缺乏 DNA 甲基化催化能力。在这里，我们表明拟南芥 DNA 甲基转移酶 2（AtDNMT2）定位于细胞核内，并与组蛋白去乙酰化有关。双分子荧光互补和下拉实验表明 AtDNMT2 与 2 型组蛋白去乙酰化酶（AtHD2s）相互作用，AtHD2s 是植物中独特的组蛋白去乙酰化酶家族。通过分析 AtDNMT2：ss-葡糖醛酸酶（GUS）融合蛋白的表达，我们证明 AtDNMT2 具有在转录水平上抑制基因表达的能力。同时，athd2c 突变体植物中 AtDNMT2 基因的表达发生改变。我们提出 AtDNMT2 可能参与组蛋白去乙酰化和植物表观遗传调控网络的活性。
