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本文引用的文献

1
CE-MS analysis of the human urinary proteome for biomarker discovery and disease diagnostics.用于生物标志物发现和疾病诊断的人类尿液蛋白质组的毛细管电泳-质谱分析。
Proteomics Clin Appl. 2008 Jul 10;2(7-8):964. doi: 10.1002/prca.200800024.
2
Should kidney tubular markers be adjusted for urine creatinine? The example of urinary cystatin C.是否应该对尿液肌酐进行肾小管标志物的调整？以尿胱抑素 C 为例。
Clin Chem Lab Med. 2009;47(12):1553-6. doi: 10.1515/CCLM.2009.341.
3
Multiple reaction monitoring cubed for protein quantification at the low nanogram/milliliter level in nondepleted human serum.立方多重反应监测法可用于定量检测非耗竭人血清中低纳克/毫升水平的蛋白质。
Anal Chem. 2009 Nov 15;81(22):9343-52. doi: 10.1021/ac901447h.
4
Pre-operative urinary cathepsin D is associated with survival in patients with renal cell carcinoma.术前尿组织蛋白酶D与肾细胞癌患者的生存率相关。
Br J Cancer. 2009 Oct 6;101(7):1175-82. doi: 10.1038/sj.bjc.6605250.
5
Discovery and validation of urinary biomarkers for prostate cancer.前列腺癌尿液生物标志物的发现与验证
Proteomics Clin Appl. 2008 Mar 7;2(4):556-570. doi: 10.1002/prca.200780082.
6
Clusterin as a diagnostic and prognostic marker for transitional cell carcinoma of the bladder.簇集蛋白作为膀胱移行细胞癌的诊断和预后标志物。
Pathol Oncol Res. 2010 Mar;16(1):101-9. doi: 10.1007/s12253-009-9196-3. Epub 2009 Sep 9.
7
Identification of Cystatin SN as a novel tumor marker for colorectal cancer.鉴定胱抑素SN作为结直肠癌的一种新型肿瘤标志物。
Int J Oncol. 2009 Jul;35(1):33-40.
8
A depletion strategy for improved detection of human proteins from urine.一种用于改进从尿液中检测人类蛋白质的耗尽策略。
J Biomol Tech. 2009 Apr;20(2):101-8.
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UHRF1 is a novel molecular marker for diagnosis and the prognosis of bladder cancer.UHRF1是一种用于膀胱癌诊断和预后的新型分子标志物。
Br J Cancer. 2009 Jul 7;101(1):98-105. doi: 10.1038/sj.bjc.6605123. Epub 2009 Jun 2.
10
The use of PCA3 in the diagnosis of prostate cancer.PCA3 在前列腺癌诊断中的应用。
Nat Rev Urol. 2009 May;6(5):255-61. doi: 10.1038/nrurol.2009.40.

尿液采集和处理用于蛋白质生物标志物的发现和定量。

Urine collection and processing for protein biomarker discovery and quantification.

机构信息

Proteomics, H. Lee Moffitt Cancer Center, Tampa, FL 33612, USA.

出版信息

Cancer Epidemiol Biomarkers Prev. 2010 Apr;19(4):953-9. doi: 10.1158/1055-9965.EPI-10-0069. Epub 2010 Mar 23.

DOI:10.1158/1055-9965.EPI-10-0069
PMID:20332277
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2852495/
Abstract

BACKGROUND

Urine is a useful source of protein for biomarker discovery and assessment because it is readily available, can be obtained by noninvasive collection methods, and enables monitoring of a wide range of physiologic processes and diseases. Urine aliquots provide enough protein for multiple analyses, combining current protocols with new techniques.

CONCLUSIONS

Standardized collection and processing protocols are now being established and new methods for protein detection and quantification are emerging to complement traditional immunoassays. The current state of urine collection, specimen processing, and storage is reviewed with regard to discovery and quantification of protein biomarkers for cancer.

摘要

背景

尿液是一种用于生物标志物发现和评估的有用蛋白质来源，因为它易于获得，可以通过非侵入性采集方法获得，并能够监测广泛的生理过程和疾病。尿液等分试样提供了足够的蛋白质用于多种分析，将当前的方案与新技术相结合。

结论

目前正在制定标准化的收集和处理方案，并且新的蛋白质检测和定量方法也在不断涌现，以补充传统的免疫测定方法。本文就癌症的蛋白质生物标志物的发现和定量方面，对尿液采集、标本处理和储存的现状进行了综述。