基质金属蛋白酶 2 和 9 在人胃癌和浅表性胃炎中的表达。
Expression of matrix metalloproteinases 2 and 9 in human gastric cancer and superficial gastritis.
机构信息
Institute of Public Health, Veracruzana University, Xalapa, Veracruz, CP 91190, Mexico.
出版信息
World J Gastroenterol. 2010 Mar 28;16(12):1500-5. doi: 10.3748/wjg.v16.i12.1500.
AIM
To assess expression of matrix metalloproteinases 2 (MMP2) and MMP9 in gastric cancer, superficial gastritis and normal mucosa, and to measure metalloproteinase activity.
METHODS
MMP2 and MMP9 mRNA expression was determined by quantitative real-time polymerase chain reaction. Normalization was carried out using three different factors. Proteins were analyzed by quantitative gelatin zymography (qGZ).
RESULTS
18S ribosomal RNA (18SRNA) was very highly expressed, while hypoxanthine ribosyltransferase-1 (HPRT-1) was moderately expressed. MMP2 was highly expressed, while MMP9 was not detected or lowly expressed in normal tissues, moderately or highly expressed in gastritis and highly expressed in cancer. Relative expression of 18SRNA and HPRT-1 showed no significant differences. Significant differences in MMP2 and MMP9 were found between cancer and normal tissue, but not between gastritis and normal tissue. Absolute quantification of MMP9 echoed this pattern, but differential expression of MMP2 proved conflictive. Analysis by qGZ indicated significant differences between cancer and normal tissue in MMP-2, total MMP-9, 250 and 110 kDa bands.
CONCLUSION
MMP9 expression is enhanced in gastric cancer compared to normal mucosa; interpretation of differential expression of MMP2 is difficult to establish.
目的
评估基质金属蛋白酶 2（MMP2）和 MMP9 在胃癌、浅表性胃炎和正常黏膜中的表达，并测量金属蛋白酶活性。
方法
通过实时定量聚合酶链反应（PCR）确定 MMP2 和 MMP9 的 mRNA 表达。采用三种不同的因素进行归一化。通过定量明胶酶谱（qGZ）分析蛋白质。
结果
18S 核糖体 RNA（18SRNA）高度表达，而次黄嘌呤核糖基转移酶-1（HPRT-1）中度表达。MMP2 高度表达，而 MMP9 在正常组织中未检测到或低度表达，在胃炎中中度或高度表达，在癌症中高度表达。18SRNA 和 HPRT-1 的相对表达无显著差异。MMP2 和 MMP9 在癌症和正常组织之间存在显著差异，但在胃炎和正常组织之间无差异。MMP9 的绝对定量反映了这种模式，但 MMP2 的差异表达证明存在矛盾。qGZ 分析表明 MMP-2、总 MMP-9、250 和 110 kDa 条带在癌症和正常组织之间存在显著差异。
结论
与正常黏膜相比，胃癌中 MMP9 的表达增强；MMP2 差异表达的解释难以确定。
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