Mrena J, Wiksten J-P, Nordling S, Kokkola A, Ristimäki A, Haglund C
Department of Surgery, Helsinki University Central Hospital, Helsinki, Finland.
J Clin Pathol. 2006 Jun;59(6):618-23. doi: 10.1136/jcp.2005.033761.
Matrix metalloproteinases (MMPs) MMP-2 and MMP-9 can degrade type IV collagen of extracellular matrix and basal membranes. As cyclo-oxygenase-2 (COX-2) has been shown to activate MMPs, creating one of the COX-2-promoted pathways of tumour growth and metastasis, the prognostic role of MMP-2 and MMP-9 in gastric cancer was assessed and their association with COX-2 expression was evaluated.
Samples were collected from 342 consecutive patients operated on for gastric cancer, of which 315 were acceptable for MMP-2, MMP-9 and COX-2 immunohistochemistry. Specimens were stained with specific antibodies, evaluated and categorised by two interpreters, and then correlated with clinical data and survival.
Epithelial MMP-2 immunoreactivity was associated with male sex, high stage, advanced penetration depth, non-curative surgery, high COX-2 expression and poor survival. Stromal MMP-2 expression correlated with high stage, intestinal type and non-curative surgery whereas MMP-9 correlated only with intestinal type. Stage, intent of surgery and COX-2 were independent prognostic factors.
Epithelial MMP-2 expression in gastric cancer is associated with aggressive forms, COX-2 and poor survival, although MMP-2 was not an independent prognostic factor. In gastric cancer tumour growth is apparently induced by COX-2, and invasion is mediated by MMP-2.
基质金属蛋白酶（MMPs）中的MMP - 2和MMP - 9可降解细胞外基质和基底膜的IV型胶原蛋白。由于环氧合酶 - 2（COX - 2）已被证明可激活MMPs，从而形成COX - 2促进肿瘤生长和转移的途径之一，因此评估了MMP - 2和MMP - 9在胃癌中的预后作用，并评估了它们与COX - 2表达的相关性。
收集了342例连续接受胃癌手术患者的样本，其中315例样本可用于MMP - 2、MMP - 9和COX - 2免疫组织化学检测。标本用特异性抗体染色，由两名解读人员进行评估和分类，然后与临床数据和生存率进行关联分析。
上皮MMP - 2免疫反应性与男性、高分期、浸润深度深、非根治性手术、高COX - 2表达及生存率低相关。基质MMP - 2表达与高分期、肠型及非根治性手术相关，而MMP - 9仅与肠型相关。分期、手术意图和COX - 2是独立的预后因素。
胃癌上皮MMP - 2表达与侵袭性形式、COX - 2及生存率低相关，尽管MMP - 2不是独立的预后因素。在胃癌中，肿瘤生长显然由COX - 2诱导，侵袭由MMP - 2介导。
