Department of Medical Radiation Physics, Lund University, SE-221 85 Lund, Sweden.
Radiat Res. 2010 Apr;173(4):433-40. doi: 10.1667/RR1733.1.
Single-fraction radiation therapy with 5 or 15 Gy (60)Co gamma radiation was combined with intraperitoneal injections of syngeneic interferon gamma (IFN-gamma)-transfected cells in rats with intracerebral N29 or N32 glioma tumors at days 7, 21 and 35 after inoculation. For intracerebral N29 tumors, single-fraction radiation therapy with 5 or 15 Gy had no significant effect on the survival time. Immunization with IFN-gamma-transfected N29 cells significantly increased the survival time by 61%. Single-fraction radiation therapy with 5 Gy combined with immunization increased the survival time significantly by 87% and complete remissions by 75% while with 15 Gy the survival time increased 45% with 38% complete remissions. For intracerebral N32 tumors, single-fraction radiation therapy with 15 Gy increased the survival time significantly by 20%. Immunization by itself had no significant effect with IFN-gamma-transfected N32 cells, but combined with 15 Gy single-fraction radiation therapy it increased survival time significantly by 40%, although there were no complete remissions. Based on these findings, we suggest a new therapeutic regimen for malignant glioma using single-fraction radiation therapy with a target absorbed dose of the order of 5-10 Gy combined with clinically verified immunotherapy.
在接种后第 7、21 和 35 天，将 5 或 15 Gy（60）Co 伽马射线单次放射治疗与腹腔内注射同源干扰素 γ（IFN-γ）转染细胞联合用于颅内 N29 或 N32 神经胶质瘤肿瘤的大鼠中。对于颅内 N29 肿瘤，单次 5 或 15 Gy 放射治疗对生存时间没有显著影响。用 IFN-γ 转染的 N29 细胞免疫接种显著将生存时间延长了 61%。单次 5 Gy 放射治疗联合免疫接种将生存时间显著延长了 87%，完全缓解率为 75%，而 15 Gy 则将生存时间延长了 45%，完全缓解率为 38%。对于颅内 N32 肿瘤，单次 15 Gy 放射治疗将生存时间显著延长了 20%。IFN-γ 转染的 N32 细胞免疫接种本身没有显著效果，但与 15 Gy 单次放射治疗联合使用可将生存时间显著延长 40%，尽管没有完全缓解。基于这些发现，我们建议使用单次剂量为 5-10 Gy 的目标吸收剂量的放射治疗联合临床验证的免疫疗法，为恶性神经胶质瘤制定一种新的治疗方案。
