BMP12 和 BMP13 基因转导诱导间充质祖细胞和前交叉韧带细胞的韧带形成分化。
BMP12 and BMP13 gene transfer induce ligamentogenic differentiation in mesenchymal progenitor and anterior cruciate ligament cells.
机构信息
Orthopedic Center for Musculoskeletal Research, Department of Orthopedic Surgery, König-Ludwig-Haus, Julius-Maximilians-University, Würzburg, Germany.
出版信息
Cytotherapy. 2010 Jul;12(4):505-13. doi: 10.3109/14653241003709652.
BACKGROUND AIMS
To date there are only very few data available on the ligamentogenic differentiation capacity of mesenchymal stromal/progenitor cells (MSC) and anterior cruciate ligament (ACL) fibroblasts.
METHODS
We describe the in vitro potential of MSC and ACL cells to undergo ligamentogenic differentiation upon transduction with adenoviral vectors encoding the human cDNA for bone morphogenetic protein (BMP) 12 and BMP13, also known as growth and differentiation factors (GDF) 6 and 7, respectively.
RESULTS
Transgene expression for at least 14 days was confirmed by Western blot analyzes. After 21 days of cell culture within collagen type I hydrogels, histochemical (hematoxylin/eosin (H&E), Azan and van Gieson), immunohistochemical and polymerase chain reaction (PCR) analyzes of the genetically modified constructs of both cell types revealed elongated, viable fibroblast-like cells embedded in a ligament-like matrix rich in collagens, vimentin, fibronectin, decorin, elastin, scleraxis, tenascin, and tenomodulin.
CONCLUSIONS
It appears that both MSC and ACL fibroblasts are capable of ligamentogenic differentiation with these factors. This information may aid in the development of biologic approaches to repair and restore ACL after injury.
背景目的
迄今为止，关于间充质基质/祖细胞（MSC）和前交叉韧带（ACL）成纤维细胞的韧带形成分化能力的数据非常有限。
方法
我们描述了 MSC 和 ACL 细胞在转导编码人骨形态发生蛋白（BMP）12 和 BMP13（分别称为生长分化因子（GDF）6 和 7）的腺病毒载体后体外发生韧带形成分化的潜力。
结果
通过 Western blot 分析证实了至少 14 天的转基因表达。在 I 型胶原水凝胶中培养细胞 21 天后，对两种细胞类型的基因修饰构建体进行组织化学（苏木精/伊红（H&E）、阿赞和范盖森）、免疫组织化学和聚合酶链反应（PCR）分析显示，长而存活的成纤维细胞样细胞嵌入富含胶原蛋白、波形蛋白、纤维连接蛋白、饰胶蛋白、弹性蛋白、腱调蛋白、腱生蛋白和腱蛋白多糖的韧带样基质中。
结论
似乎这两种 MSC 和 ACL 成纤维细胞都具有这些因子的韧带形成分化能力。这些信息可能有助于开发修复和重建 ACL 损伤后的生物学方法。
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