Centre de Biophysique Moléculaire, CNRS UPR4301, Université d'Orléans and Inserm, rue Charles Sadron, 45071 Orléans Cedex 2, France.
Biomaterials. 2010 Jul;31(19):5237-45. doi: 10.1016/j.biomaterials.2010.02.077. Epub 2010 Mar 23.
We report the ability of amino- and carboxyl-modified MCM-41 mesoporous silica nanoparticles (MSN) to deliver gene in vivo in rat Achilles tendons, despite their inefficiency to transfect primary tenocytes in culture. We show that luciferase activity lasted for at least 2 weeks in tendons injected with these MSN and a plasmid DNA (pDNA) encoding the luciferase reporter gene. By contrast, in tendons injected with naked plasmid, the luciferase expression decreased as a function of time and became hardly detectable after 2 weeks. Interestingly, there were neither signs of inflammation nor necrosis in tendon, kidney, heart and liver of rat weekly injected with pDNA/MSN formulation during 1.5 months. Our main data concern the acceleration of Achilles tendons healing by PDGF-B gene transfer using MSN. Biomechanical properties and histological analyses clearly indicate that tendons treated with MSN and PDGF gene healed significantly faster than untreated tendons and those treated with pPDGF alone.
我们报告了氨基和羧基修饰的 MCM-41 介孔硅纳米粒子（MSN）在体内向大鼠跟腱中递送基因的能力，尽管它们在培养的原代肌腱细胞中转染效率不高。我们表明，即使在向腱内注射携带编码荧光素酶报告基因的质粒 DNA（pDNA）的这些 MSN 后，荧光素酶活性至少持续了 2 周。相比之下，在向腱内注射裸质粒的情况下，荧光素酶的表达随时间呈下降趋势，在 2 周后几乎无法检测到。有趣的是，在 1.5 个月内每周向大鼠注射 pDNA/MSN 制剂，在肌腱、肾脏、心脏和肝脏中均未出现炎症或坏死的迹象。我们的主要数据涉及使用 MSN 加速 PDGF-B 基因转移对跟腱愈合的影响。生物力学性能和组织学分析清楚地表明，用 MSN 和 PDGF 基因处理的肌腱比未处理的肌腱以及单独用 pPDGF 处理的肌腱愈合得更快。
