组氨酸功能化介孔二氧化硅纳米颗粒改善基因传递
Improved gene transfer with histidine-functionalized mesoporous silica nanoparticles.
作者信息
Brevet David, Hocine Ouahiba, Delalande Anthony, Raehm Laurence, Charnay Clarence, Midoux Patrick, Durand Jean-Olivier, Pichon Chantal
机构信息
Institut Charles Gerhardt Montpellier, UMR 5253 CNRS-UM2-ENSCM-UM1, CC1701 Equipe Chimie Moléculaire et Organisation du Solide, Place Eugène Bataillon, Cedex 05, Montpellier 34095, France.
Centre de Biophysique Moléculaire, CNRS-UPR 4301, rue Charles Sadron, Orléans 45071, France.
出版信息
Int J Pharm. 2014 Aug 25;471(1-2):197-205. doi: 10.1016/j.ijpharm.2014.05.020. Epub 2014 May 20.
Mesoporous silica nanoparticles (MSN) were functionalized with aminopropyltriethoxysilane (MSN-NH2) then L-histidine (MSN-His) for pDNA delivery in cells and in vivo. The complexation of pDNA with MSN-NH2 and MSN-His was first studied with gel shift assay. pDNA complexed with MSN-His was better protected from DNase degradation than with MSN-NH2. An improvement of the transfection efficiency in cells was observed with MSN-His/pDNA compared to MSN-NH2/pDNA, which could be explained by a better internalization of MSN-His. The improvement of the transfection efficiency with MSN-His was also observed for gene transfer in Achilles tendons in vivo.
介孔二氧化硅纳米颗粒（MSN）先用氨丙基三乙氧基硅烷进行功能化处理（MSN-NH2），然后用L-组氨酸进行功能化处理（MSN-His），用于细胞内和体内的pDNA递送。首先通过凝胶迁移试验研究了pDNA与MSN-NH2和MSN-His的络合情况。与MSN-NH2相比，与MSN-His络合的pDNA对DNase降解的保护效果更好。与MSN-NH2/pDNA相比，观察到MSN-His/pDNA在细胞中的转染效率有所提高，这可以通过MSN-His更好的内化作用来解释。在体内跟腱的基因转移中也观察到了MSN-His转染效率的提高。
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