厚朴酚对金黄色葡萄球菌α-毒素分泌的影响。
Influence of magnolol on the secretion of alpha-toxin by Staphylococcus aureus.
机构信息
Institute of Zoonoses, College of Animal Sciences and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China.
出版信息
Molecules. 2010 Mar 12;15(3):1679-89. doi: 10.3390/molecules15031679.
In this study we investigated the antimicrobial activity of magnolol on Staphylococcus aureus. The minimal inhibitory concentrations of magnolol against 31 S. aureus strains ranged from 4-32 microg/mL. In addition, hemolysin assays, Western blotting, and real-time RT-PCR were performed to investigate the effect of magnolol on alpha-toxin secretion by both methicillin-sensitive S. aureus (MSSA) and methicillin-resistant S. aureus (MRSA). The results indicated that sub-inhibitory concentrations of magnolol dose-dependently inhibited the transcription of hla (the gene encoding alpha-toxin) in S. aureus, resulting in a reduction of alpha-toxin secretion and, thus, hemolytic activities.
在这项研究中，我们研究了厚朴酚对金黄色葡萄球菌的抗菌活性。厚朴酚对 31 株金黄色葡萄球菌的最小抑菌浓度范围为 4-32 微克/毫升。此外，还进行了溶血试验、Western blot 和实时 RT-PCR，以研究厚朴酚对甲氧西林敏感金黄色葡萄球菌（MSSA）和耐甲氧西林金黄色葡萄球菌（MRSA）的α-毒素分泌的影响。结果表明，亚抑制浓度的厚朴酚剂量依赖性地抑制了金黄色葡萄球菌中 hla（编码α-毒素的基因）的转录，导致α-毒素分泌减少，从而降低溶血活性。
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