Control of Foodborne by Shikonin, a Natural Extract.

Foodborne () has attracted widespread attention due to its foodborne infection and food poisoning in human. Shikonin exhibits antibacterial activity against a variety of microorganisms, but there are few studies on its antibacterial activity against . This study aims to explore the antibacterial activity and mechanism of shikonin against foodborne . The results show that the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) of shikonin were equal for all tested strains ranging from 35 μg/mL to 70 μg/mL. Shikonin inhibited the growth of by reducing intracellular ATP concentrations, hyperpolarizing cell membrane, destroying the integrity of cell membrane, and changing cell morphology. At the non-inhibitory concentrations (NICs), shikonin significantly inhibited biofilm formation of which was attributed to inhibiting the expression of and genes. Moreover, shikonin also markedly inhibited the transcription and expression of virulence genes ( and ) in . In addition, shikonin has exhibited antibacterial ability against both planktonic and biofilm forms of . Importantly, in vivo results show that shikonin has excellent biocompatibility. Moreover, both the heat stability of shikonin and the antimicrobial activity of shikonin against were excellent in food. Our findings suggest that shikonin are promising for use as a natural food additive, and it also has great potential in effectively controlling the contamination of in food and reducing the number of illnesses associated with .