Institute for Coastal Research, Department for Marine Bioanalytical Chemistry, GKSS Research Centre Geesthacht, Max-Planck-Str, 21502 Geesthacht, Germany.
Mar Biotechnol (NY). 2010 Jun;12(3):308-17. doi: 10.1007/s10126-010-9261-7. Epub 2010 Mar 25.
Jellyfish have an increasing impact on marine ecology. Cnidocysts bearing stinging cells afford, amongst others, prey capture and defence. Several different types of stinging capsules are found in one species and they are supposed to have specific functions, e.g. paralysing prey or adhering to it. Due to these assumed different roles of the capsules, it is suggested that toxins, which are contained in the capsules, differ in composition. Analysis of distinct types of nematocysts requires an appropriate method for the separation of the different types. Mixtures of types of nematocysts were obtained of two species of jellyfish, Aurelia aurita and Cyanea lamarckii, by maceration of the tissue. These mixtures were treated with a method called laser microdissection and pressure catapulting (LMPC). Optimized maceration methods, which were firstly introduced as a method for this purpose, in conjunction with optimized LMPC parameters lead to sufficient amounts of separated capsules of individual types for subsequent mass-spectrometric analyses. In case of A. aurita, the resulting mass spectra had some constituents in common, whereas in the overall pattern, the two distinct nematocyst types differed.
水母对海洋生态系统的影响越来越大。刺细胞囊携带刺细胞，除其他功能外，还用于捕食和防御。在一个物种中发现了几种不同类型的刺胞囊，它们被认为具有特定的功能，例如使猎物瘫痪或附着在猎物上。由于这些囊具有不同的作用，因此推测其中包含的毒素在组成上有所不同。分析不同类型的刺胞需要一种适当的方法来分离不同类型的刺胞。通过组织的研磨，从两种水母（Aurelia aurita 和 Cyanea lamarckii）中获得了混合类型的刺胞囊。将这些混合物用一种称为激光显微切割和压力弹射（LMPC）的方法进行处理。优化的研磨方法，最初被引入作为一种用于此目的的方法，与优化的 LMPC 参数相结合，导致足以分离出各个类型的分离刺胞囊，以便进行后续的质谱分析。在 A. aurita 的情况下，得到的质谱具有一些共同的成分，而在整体模式中，两种不同的刺胞囊类型有所不同。
